Organotypic cultures and Fura-2

Chris Hatton CD.Hatton26 at bigfoot.com
Fri Aug 23 13:19:11 EST 1996

Dear all,

I have a question needing intellectual thought and practical experience,

I'm working with organotypic cultures, and specifically using them with
Fura-2.  We have problem with membranes absorbing at 340nm, any
suggestions how to get round this would be most appreciated.  Any way of
not using membranes would be even better, the cultures are brain, but
other parts of body also welcome.  For those interested I'm looking at
glutamate toxicity.

Please reply either by Email or, to discussion group.


Hatton at IfN-magdeburg.de

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