I was hoping to get some help from someone out there. I am
expressing the aryl hydrocarbon (Dioxin) receptor in insect
cells and want to study its binding to a number of known
ligands and potential ligands in complex mixtures. To check
the activity of the expressed protein through the Ni-NTA
purification scheme I was hoping to use radiolabelled TCDD.
However, I am having extreme difficulty obtaining any. I would
prefer to work with tritiated or 14-C labelled 2,3,7,8-TCDD
rather than radioiodinate as some labs do. Can anyone help me
get a hold of some ?
