Anil K Dhiri <pathit at pathit.com> wrote:
>Colleagues
>>I am lookinto into apoptosis and would be keen to hear from individuals
>who have used an immunochemical marker to identify apoptotic bodies in
>the liver.
>>Anil
>>>>>>>>>>>>>>>>>>>>>>>>>>Dr Anil K Dhiri, DVM,MBA
>http://www.pathit.com>Pathology On-Line
><<<<<<<<<<<<<<<<<<<<<<<<
Hi,
We've used ApoTag from Oncor on formalin fixed rat and mouse liver. It
didn't work on frozen liver at all. There is a good article by Tom
Goldsworthy in Toxicologic Pathology 24(1):24-35, 1996, comparing
different methods for measuring apoptosis in liver. We've also used an
ELISA kit from Boehringer with some success.
Good luck,
Angela
