In article <5ijkvh$jk0$1 at cronkite.seas.gwu.edu>, mnrao at gwis2.circ.gwu.edu
says...
>>Hello Everyone,
>Does anyone in this group know an efficient method to obtain the different
>cells that make up the liver, such as hepatocytes, lipocytes, kupfer
>cells, etc. Please let me know.
>>Thanks in advance.
>>--
>*******************************
>Manjunath N.Rao
>*************************
Hi. I think it is an interresting topic in view of characterising cell
type specific genetic damage. One method could be to hierarchically sort cells
through flow cytometry (Facs). This however requires that easily
detectable selective markers can be used for each cell type. It also requires
much work, since there could be overlaps between individual markers.
I would be interrested in comments about this approach too.
Kind regards
Michael Sjögren, PhD toxicology
