Whoops! Make that 0.1% MS222 (dissolved in 0.2% sodium bicarb.).
Pura Vida,
Stan
Stanley Sessions PhD
Department of Biology
Hartwick College
Oneonta, NY 13820-1904
607-431-4764
sessionss from hartwick.edu
On Apr 2, 2010, at 11:09 AM, Stanley Sessions wrote:
> Axolotls (and other salamanders) do not appear to need a post-
> amputation analgesic (other than, perhaps, keeping them cold for a
> while). My assay is feeding: they usually commence feeding, as if
> nothing happened, as soon as they recover from the anesthetic. I
> don't think that would happen if they were in severe post-amputation
> pain.
>> I have found nothing that comes close to MS222 for safely
> anesthetizing axolotls or any salamander or fish (we use 0.3% for
> newts). It is reasonably rapid (takes 15-20 minutes for adult
> newts), gentle yet profound and long-lasting, with a steep recovery
> curve. However, plain MS222 dissolved in water has a very low pH
> which can cause significant discomfort to the animal (judging by the
> fact that they thrash around). So I recommend buffering it (e.g.
> with 0.2% sodium bicarbonate). Another advantage of (buffered) MS222
> is that you can make a large quantity of it and keep it in the
> refrigerator. Warm to ambient temperature (to match the water that
> the animal is in) before using, however.
>> Pura Vida,
>> Stan
> Stanley Sessions PhD
> Department of Biology
> Hartwick College
> Oneonta, NY 13820-1904
> 607-431-4764
>sessionss from hartwick.edu>>>>>>>> On Apr 18, 2009, at 4:07 AM, Jordi Cantó wrote:
>>> Hello Natalie,
>>>> Please, can I have the summary of responses to these questions:
>>>> 1) Do axolotls need a post leg amputation analgesic? If not why
>> not? If they do, what sort is appropriate? For how long into the
>> regeneration period do they require painkillers?
>>>> 2) Is there another anesthetic other than MS222 appropriate for
>> anesthetising the axolotls for such an amputation?
>>>> Thanks,
>>>> --
>> Jordi Cantó Martorell
>> Director Servei d'Estabulari - SIAL
>> Universitat Autònoma de Barcelona
>> SPAIN
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