From Patricia.Kite from agnews.dds.ca.gov Wed Jul 9 17:30:04 2008 From: Patricia.Kite from agnews.dds.ca.gov (Patricia Kite) Date: Wed Jul 9 18:05:34 2008 Subject: [Yeast] yeast division numbers needed Message-ID: If I start out with one yeast cell in a comfortable environment, roughly how many yeast cells will there be in that environment in one hour? 10 hours. I have received answers from total number of 6 or "a single cell can grow into a barely visible colony in one day." need a short numerical answer for a project. thank you -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/yeast/attachments/20080709/3c24abe2/attachment.html From Gael.Yvert from ens-lyon.fr Thu Jul 10 02:26:42 2008 From: Gael.Yvert from ens-lyon.fr (=?ISO-8859-1?Q?Ga=EBl_Yvert?=) Date: Thu Jul 10 08:50:50 2008 Subject: [Yeast] yeast division numbers needed In-Reply-To: References: Message-ID: <4875B9B2.6020007@ens-lyon.fr> Hey Patricia, For diploid cerevisiae you can count on a 50 (rich medium) to 85 (minimal medium) minutes generation time (gt) at 30?C. Then your number goes as 2^(time/gt). Good luck with the project, Gael. Patricia Kite a ?crit : > If I start out with one yeast cell in a comfortable environment, > roughly how many yeast cells will there be in that environment in one > hour? 10 hours. > > I have received answers from total number of 6 or > "a single cell can grow into a barely visible colony in one day." > > > need a short numerical answer for a project. > > thank you > ------------------------------------------------------------------------ > > _______________________________________________ > Yeast mailing list > Yeast@net.bio.net > http://www.bio.net/biomail/listinfo/yeast From linnea.hedin from cbr.su.se Thu Jul 17 04:31:33 2008 From: linnea.hedin from cbr.su.se (Linnea Hedin) Date: Thu Jul 17 10:05:46 2008 Subject: [Yeast] Plasmid available for expressing only TAP-tag as control? Message-ID: Dear all, I'm trying to find out if there's a plasmid expressing the TAP-tag available. As far as I understand, the plasmids sold by Euroscarf are only for tagging proteins and do not replicate in yeast. Has anyone heard of a commercial plasmid for expressing only the TAP-tag? Preferably the expression should not need to be induced. I need it for a control purification. Thanks in advance! Kind regards, Linnea Hedin - - - PhD Student Center for Biomembrane Research Stockholm University 106 91 Stockholm, Sweden linnea.hedin@cbr.su.se, linnea.hedin@dbb.su.se -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/yeast/attachments/20080717/8ced8f31/attachment.html From shoutaka from vos.nagaokaut.ac.jp Thu Jul 17 06:39:13 2008 From: shoutaka from vos.nagaokaut.ac.jp (shoushou) Date: Thu Jul 17 10:05:51 2008 Subject: [Yeast] get of ura3 mutant Message-ID: <18506549.post@talk.nabble.com> I tried to get an ura3 mutant of a yeast by EMS or MNNG mutagenesis and 5-FOA selection. But I could not get it. So I would like to get it by other method. Could you tell me other methods that create ura3 mutant of yeast. -- View this message in context: http://www.nabble.com/get-of-ura3-mutant-tp18506549p18506549.html Sent from the Bio.net - Yeast mailing list archive at Nabble.com. From neural.nerd from gmail.com Fri Jul 18 13:44:10 2008 From: neural.nerd from gmail.com (Roy Buchanan) Date: Fri Jul 18 16:02:26 2008 Subject: [Yeast] get of ura3 mutant In-Reply-To: <18506549.post@talk.nabble.com> References: <18506549.post@talk.nabble.com> Message-ID: <18535542.post@talk.nabble.com> Is it diploid or haploid? What about using homologous recombination of a PCR generated cassette? shoushou wrote: > > I tried to get an ura3 mutant of a yeast by EMS or MNNG mutagenesis and > 5-FOA selection. But I could not get it. So I would like to get it by > other method. Could you tell me other methods that create ura3 mutant of > yeast. > -- View this message in context: http://www.nabble.com/get-of-ura3-mutant-tp18506549p18535542.html Sent from the Bio.net - Yeast mailing list archive at Nabble.com. From shoutaka from vos.nagaokaut.ac.jp Mon Jul 21 18:55:48 2008 From: shoutaka from vos.nagaokaut.ac.jp (shoushou) Date: Tue Jul 22 15:15:35 2008 Subject: [Yeast] get of ura3 mutant In-Reply-To: <18535542.post@talk.nabble.com> References: <18506549.post@talk.nabble.com> <18535542.post@talk.nabble.com> Message-ID: <18579918.post@talk.nabble.com> I am not sure whether it is diploid or haploid, but I think that it may be diploid. Could you teach me in more detail the method using homologous recombination of a PCR generated cassette. Roy Buchanan wrote: > > Is it diploid or haploid? What about using homologous recombination of a > PCR generated cassette? > > > > shoushou wrote: >> >> I tried to get an ura3 mutant of a yeast by EMS or MNNG mutagenesis and >> 5-FOA selection. But I could not get it. So I would like to get it by >> other method. Could you tell me other methods that create ura3 mutant of >> yeast. >> > > -- View this message in context: http://www.nabble.com/get-of-ura3-mutant-tp18506549p18579918.html Sent from the Bio.net - Yeast mailing list archive at Nabble.com. From phoebegibson__ from hotmail.com Wed Jul 23 04:08:29 2008 From: phoebegibson__ from hotmail.com (Phoebe Gibson) Date: Wed Jul 23 12:18:50 2008 Subject: [Yeast] Anaerobic Respiration in Yeast Message-ID: What is average rate of respiration for yeast? How does temperature affect this rate? What specific enzymes are most affected by temperature changes? _________________________________________________________________ It's simple! 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