[Yeast] get of ura3 mutant

[Roy Buchanan]

Is it diploid or haploid? What about using homologous recombination of a PCR
generated cassette?

 

shoushou wrote:
> 
> I tried to get an ura3 mutant of a yeast by EMS or MNNG mutagenesis and
> 5-FOA selection. But I could not get it. So I would like to get it by
> other method. Could you tell me other methods that create ura3 mutant of
> yeast.
> 

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