>From The Worm Breeder's Gazette, 1996, v. 14, No. 4, p. 50.
AN ATTEMPT TO SLOW AGING IN C. elegans. 5.THE EFFECT OF
ROYAL JELLY EXTRACT WATER SOLUIONS IN DIFFERENT
CONCENTRATIONS
Vladimir V. Bakaev, box 45, Novosibirsk, 630107, USSR
The purpose of this study was the effect of different concent-
rations of royal jelly extract on nematode life span. In this
experiment royal jelly extract was used in the form of "Apilac-
tosa" (produced by "Fertane", Moscow, Nagatinskaya str., 1) in
following dilutions : 1:10(3), 1:10(4), 1:10(5), 1:10(6),
1:10(7), 1:10(8), 1:10(9) and 1:10(10). Three adult animals (3
- 5 days old) were kept in microtitre wells containing 0,75 ml
of liquid medium (with E. coli and without royal jelly extract)
during 4 hours, then they were discarded and newborn larvae we-
re transferred in next wells (with royal jelly extract in any
concentration) every day (one worm in one well). This investi-
gation was carried out in temperature +21oC and in the dark-
ness. The obtained results are presented in the following tab-
le.
───────────────────────┬─────┬──────────────────────────────
Concentration of royal│ │ Longevity (days)
│ ├──────────────┬───────────────
jelly extract │ n │ mean+/-S.D. │ maximal
───────────────────────┼─────┼──────────────┼───────────────
1:10(3) │ 12 │ lethal
│ │ │
1:10(4) │ 11 │ 18,2+/-1,8 │ 28
│ │ │
1:10(5) │ 12 │ 19,2+/-2,2 │ 32
│ │ │
1:10(6) │ 12 │ 19,3+/-1,7 │ 28
│ │ │
1:10(7) │ 12 │ 15,8+/-2,2 │ 28
│ │ │
1:10(8) │ 11 │ 20,6+/-3.1 │ 32
│ │ │
1:10(9) │ 8 │ 12,3+/-3,5 │ 34
│ │ │
1:10(10) │ 12 │ 17,5+/-2,3 │ 31
───────────────────────┴─────┴──────────────┴───────────────
Conclusion: If the extract from royal jelly was applied to C.
elegans during the whole life span in above described conditi-
ons, the most effective concentration was 1:10(8). It seemed
for me more 7 0reasonable to study the effect on longevity of any
concentrations near 1:10(8).
Acknowledgment: The author wishes to express his thanks to CGC
for providing C. elegans (Bristol, N2) and E. coli OP50.
