Nature Article: Apoptosis

Aubrey de Grey ag24 at mole.bio.cam.ac.uk
Tue Jan 4 11:32:42 EST 2000

Paul Brookes wrote:

> The assumption by everyone has been that what they're measuring is
> apoptosis.  What they in fact measure is cell viability by Trypan Blue
> exclusion.  No TUNEL staining, DNA laddering, or caspase activation.
> The WT cells shown in Fig 2C don't look particularly apoptotic.   Thus
> it could be apoptosis, necrosis, or anything on the spectrum in between.

Very good point -- but in fact they do mention TUNEL staining (page 310,
left column, line 3).

> The notion that "apoptosis is something to be prevented in order to
> prolong lifespan" is certainly not proven by this paper.

I absolutely agree.

> What they have neatly shown is that prevention of cell death and
> prolonged lifespan are linked, which isn't exactly a great leap of
> faith for anyone.

Hm, well it surprised me!

> By the way .... do MEF's have many mitochondria?

I don't know.

Aubrey de Grey

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