anesthetizing earthworms

angel de leon jadeleon at
Mon Apr 15 10:45:08 EST 1996

Dear Sandy,

   Just I return from my spring vacations, and I found your message about
anesthetizing earthworms. In my class we put big earthworms (150-200 mm)wrap
in blotting paper, but the worm must be stright. When the specimens are
ready they are placed inside a hermetic cristal box, and are put a cotton
damp with chloroform, for not less than 10 minutes. The specimens may be are
dead in that time, and the study of the inner organs are ready.

Angel de Leon
Lab. Zoologia de Invertebrados
Fac. Ciencias Biologicas, U.A.N.L.
Ap. Postal 5, Suc."F"
San Nicolas de los Garza, N.L.
66451 MEXICO
e-mail: jadeleon at

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