[Arabidopsis] Semi Solid Agarose Library Amplification

[Holt, Ben F. III]

Hi Everyone,

Last week I requested protocols for semi-solid agarose amplification of cDNA libraries. Scott Michaels and Jean Danyluk were both nice enough to dig out the company manuals with those protocols (and both even directed me to the page number!). Several others wrote to me asking for the same information, so I cut the pages from those Gibco and Stratagene  manuals and created a single PDF document - it's attached.

Enjoy and thanks again Jean and Scott.

Ben

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Ben Holt
Assistant Professor
University of Oklahoma
Department of Botany and Microbiology
GLCH Rm 219
770 Van Vleet Oval
Norman, OK  73019
Phone (405)325-9018
FAX   (405)325-7619
http://www.ou.edu/cas/botany-micro/faculty/holt.html