Hi Jon,
It might be due to multiple factors like a change in the chamber temperature, over bleaching of the seed during sterilization or due to insects.
Nik
Sent from my iPhone
> On Dec 15, 2015, at 12:09 PM, "arab-gen-request from oat.bio.indiana.edu" <arab-gen-request from oat.bio.indiana.edu> wrote:
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>>> Today's Topics:
>> 1. chlorotic seedlings (Jon Monroe)
>>> ----------------------------------------------------------------------
>> Message: 1
> Date: Fri, 11 Dec 2015 14:57:54 -0500
> From: Jon Monroe <monroejd from jmu.edu>
> Subject: [Arabidopsis] chlorotic seedlings
> To: <arab-gen from magpie.bio.indiana.edu>
> Message-ID: <566B2AC2.9040907 from jmu.edu>
> Content-Type: text/plain; charset="utf-8"; format=flowed
>> Hi all,
>> We are experiencing a problem with our Arabidopsis seedlings becoming
> chlorotic a few days after being transplanted (at a week after planting)
> from Sunshine mix soil into the same soil supplemented with standard
> nutrient solution. Some seedlings remain chlorotic and stunted but
> others recover and then look fine. We've made all new nutrient stocks
> and the problem persists. We also changed bales of soil. We've been
> growing them this way for years without problems. Any ideas? Thanks.
>> Jon
>> --
> Jonathan Monroe, Professor
> Department of Biology
> 3028-B Bioscience
> 951 Carrier Dr. MSC 7801
> James Madison University
> Harrisonburg, VA 22807
> 540-568-6649, http://www.jmu.edu/biology/>>>> ------------------------------
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