vectors

U6525069 at ucsvc.ucs.unimelb.edu.au U6525069 at ucsvc.ucs.unimelb.edu.au
Thu Jun 20 17:47:00 EST 1991


Dear Network,
We have isolated a mutant which is sensitive to cadmium. The phenotype
is expressed in callus tissue and would aqppear to be a reasonable 
candidate for cloning by complementation using a library in a binary    
vector constructed either from YACs in the region of the gene or DNA of
the appropriate size identified using nearby RFLPs and purified from a 
CHEF gel. Has anyone tried this approach for another selectable gene? Can
anyone suggest a useful vector, preferably not subject to proprietary
rights, for this approach? I would be grateful for any advice, please.
Chris Cobbett.



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