Sun Feb 26 08:09:29 EST 1995
I am looking for a reducing agent which should not destroy GUS
(B-Glucuronidase, pH 7.0 should be maintained), but prevent plant tissues
(alfalfa) from oxidation (turning into brown) when they are sliced (on the
vibrotome). Any suggestions.
I also need to know how GUS stained plant material can embedded for
sectioning. I am actually looking for a precedure for dehydration,
sectioning in order to visualize GUS staining.
Thank you very much for help.
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