in situ hybridization/fixing of sections to glass slides

olet at unigen.unit.no olet at unigen.unit.no
Tue Feb 28 13:45:43 EST 1995


In article <9502172125.AA22746 at pilot1.cl.msu.edu> peterswi at PILOT.MSU.EDU ("Wilfried Peters") writes:
>From: peterswi at PILOT.MSU.EDU ("Wilfried Peters")
>Subject: in situ hybridization/fixing of sections to glass slides
>Date: 17 Feb 1995 13:25:18 -0800

>I am doing in situ hybridization with paraffin embedded plant sections. During
>the hybridization step with a 50% formamide solution most of the sections fall
>off. Therefore I would like to fix the sections to the glass slides according
>to the method described in Tourtelotte et al. (1987), where aminopropyl-
>triethoxysilane precoated slides are activated with glutaraldehyde for covalent
>binding of the sections.
>Does anybody have experience with this fixation method using plant tissue
>sections ?
>Alternatively, does anybody have experience with commercially sold precoated
>slides ?
>Any suggestions would kindly be appreciated.

>Wilfried Peters
>peterswi at pilot.msu.edu



Yes, it works with silane/glutaraldehyde. I haven't used it a lot (Brassica, 
radicles/cotyledons/root), but the sections stick to the slides. 





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