sad and mad cloning

[XuHu]

Dear Netreader:
    We are having a great difficulty in cloning lambda genomic fragments(
one 4.5 kb and one 14 kb) into pBluescript xho 1 site.  We grew the white
transformants and checked inserts by restriction digest.  We found all of
them (from both fragments cloning) are deleted pBS vector (about 2.2 kb)
without inserts.  Check ligation there was shiftup on gel compared with
self-ligated pBS. The gene we are cloning may involve in cell division of a
plant.  Can anyone give some suggestion or comments.
                                                     Xu Hu
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