GFP detection...correction

Carrington CARRINGTON at BIO.TAMU.EDU
Sat Nov 11 13:56:13 EST 1995


Sorry, folks, but I transposed the numbers on the wavelengths for the Olympus 
BX50 U-MNU filter set in my last message.  The correct features of this 
filter set are:

Dichroic mirror:  DM400
Exciter filter:   BP360-370 (not 360-730 as was given in last post)
Barrier filter:		 BA420

Again, this filter set is useful for quick scanning of bomabarded tissue 
expressing GFP because the intensity of fluorescence is much higher than with 
the typical FITC filter sets.  Beware of the photobleaching problem, however.

Jim

James C. Carrington
Department of Biology
Texas A&M University
College Station, TX  77843
Phone: 409-845-2325
Fax: 409-845-2891
e-mail: carrington at bio.tamu.edu



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