end-labelling PCR with SSLP markers

wxd10 at PO.CWRU.EDU wxd10 at PO.CWRU.EDU
Tue Oct 31 13:54:47 EST 1995


Dear netters:
I am mapping a mutant with SSLP markers, I want to resolve 177bp and 179bp polymorphism.
Has anyone done the end-labelling PCR with SSLP markers and analyze on 
the 6% acryamide gel?  I want to know how much primer, isotope and enzyme is 
needed and the reaction conditions.
Any help will be greatly appreciated.

Weiqin Dong
Dept. of Biology 
CWRU
USA




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