organelle isolation

Tej Kothari tikothari at
Wed Oct 2 12:58:22 EST 1996

I am trying to isolate chloroplast, mitocondria and nuclei from leaf 
tissue.  The protocols I possess seem to work fairly well for chloro. 
and mito. isolation.  However, my nucleur fraction looks herendous.  I 
would appreciate it if someone could send me a good nuclei isolation 
prep and also a tested and effective chloro/mito prep.
								Thank you.

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