Enriching T-DNA tagged lines for the T-DNA insertions
gfv94001 at uconnvm.uconn.edu
Wed Oct 16 18:09:31 EST 1996
Does anyone have a protocol/reference for enriching T-DNA tagged lines for
T-DNA? Screening 7000 T-DNA tagged lines (Feldmann collection) by PCR would
be much easier if I could eliminate extraneous DNA from my genomic DNA preps.
My only lead is on a biotin/streptavadin system, where I would bind a
portion of the T-DNA gene to denatured genomic DNA containing the T-DNA tags.
This would then be coupled to streptavadin beads, and purified. If anyone
has any comments, or have found a better way to enrich T-DNA tagged lines,
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