Arabidopsis microsome prep

Doug Ormrod dpo at
Thu Jun 12 11:44:13 EST 1997

Dear Arabinauts,

I'm having problems getting microsomal proteins from Arabidopsis--most
protocols require 10-20 grams of leaf tissue, which we all know is quite
difficult with Arabidopsis!!

Does anyone have a protocol for a microsome preparation using smallish
amounts (0.5-1.0 gram) of Arabidopsis leaf and stem tissue?  I would greatly
appreciate the help!


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