Seed selection after transformation

Keiper Keipern at esvax.es.dupont.com
Tue Dec 8 14:46:11 EST 1998


I'm looking for protocols for the sterilization of A.t. seeds harvested
after a vacuum infiltration with agro.  I have been sterilizing with 50%
bleach/0.02%trition x-100 for 7 mins, but have been getting a lot of
contamination on my plates.  Any suggestions?
Thanks,
Natalie Keiper-Hrynko




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