total protein extraction/and from Arab. cultured cells

David Hunt dl.hunt at virgin.net
Fri Jul 16 17:45:18 EST 1999


I've not done it myself but i know people in my lab use a French Press in
the same way as you would use for bacterial proteins

Christophe Pical wrote:

> .
>
> Since someone is asking...
> I have tried unsuccessfully to extract total proteins from Arab cultured
> cells. I am looking for a protocol that does not involve mortar
> grinding, because my samples are 32P-labelled and in addition I want
> quantitatively reproducible results.
>
> Christophe




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