toxic constructs

Joann Conner jaconner at tifton.cpes.peachnet.edu
Thu Feb 17 08:30:19 EST 2000


Hi netters,

I am trying to make constructs for protein/protein interaction
studies and finding out that my constructs are
either accumulating point mutations or that the bacteria will not
grow well.  I have tried changing into other
common bacterial strains without luck.  I can not get my gene
cloned in pSPUTK from stratagene and
cannot TA clone it into Invitrogen's TA cloning vector.  If you have
had a similar problem, I would like to
know what vector has worked well for you.

Thanks.   joann conner
jaconner at tifton.cpes.peachnet.edu



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