Chloroplast isolation

Yube YAMAGUCHI yu-yama at
Sat Jan 15 15:42:43 EST 2000

Hello everybody,

I am trying to isolate chloroplasts from A. thaliana.
I read many protocols, but they said something different.
Now, I have two questions.
What gravity do you use at first centrifugation to get intact chloroplasts?
After isolation, does the chlorophyll content correlate with the proteine
content of stroma?

Thanks a lot!

Yube Yamaguchi
Reserch and Education Center for Genetic Information
Nara Institute of Science and Technology
8916-5 Takayama, Ikoma, Nara 630-0101, Japan

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