Second selectable marker for transformation

Frederik Börnke ricky_boernke at gmx.net
Thu Jan 20 15:19:37 EST 2000



JOSE LUIS CARRASCO JIMENEZ schrieb:
>
>     Dear colleagues,
>
>     I am interested in transforming a tobacco line
>  which is already transgenic for a construct bearing
>  a kanamycin resistance gene as a selectable marker,
>  and would highly appreciate any hint, advice or
>  comment on what alternative selectable marker
>  and vector I could use for the second transformation.
>
>     Thanks in advance.
>
>     Cheers,
>
>     Jose L. Carrasco

Hi Jose,

AFAIK we use hygromycin as a second selectable marker for
supertransformation. However, it doesn't work very well.

Ricky

*******************************************************************

Frederik Boernke
Research Group of  Molecular Plant Physiology
Institute for Plant Genetics and Crop Plant Research (IPK)
Corrensstr. 3
06466 Gatersleben
Tel.  039482 -5 321
Fax. 039482 -5 515
http://www.ipk-gatersleben.de





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