[Fwd: Re: Growing Arabidopsis in sand]
nobody at hgmp.mrc.ac.uk
Thu May 3 09:05:06 EST 2001
-------- Original Message --------
Subject: Re: Growing Arabidopsis in sand
Date: Wed, 02 May 2001 21:02:30 +0100
=46rom: Jim Tokuhisa <tokuhisa at ice.mpg.de>
Reply-To: tokuhisa at ice.mpg.de
To: Wendy Allan <wallan at uoguelph.ca>
References: <3AEEFB26.EFA193B8 at uoguelph.ca>
There are several potential stresses in your growth conditions. The
may be too wet and be causing anaerobiosis. The algae may be creating
toxins that are affecting the Arabidopsis. Air exchange might be
causing excessive humidity and heat or altered gas levels.
An alternative substrate is porous clay or ceramics. In Germany we have
been using Seramis.
I need to go, but I will send this partial message and continue it
tomorrow. Good luck.
Wendy Allan wrote:
>=20 I am trying to grow Arabidopsis cv columbia in sand. I want to be
>=20 able to quickly and easily harvest the roots for analysis. Anyways, th=
>=20 plants are germinated and grown in deep-dish petri plates filled with
>=20 moist sand under short-day conditions in a growth chamber. I fertilize
>=20 the plants frequently (ie approx. every other day) with Hoaglund's
>=20 solution. The petri plates are covered. I am having trouble with algae
>=20 growth. Also, I want the rosettes to grow large. So far, after 2 to 3
>=20 weeks, the rosettes become completely chlorotic. I am also growing abou=
>=20 10-12 plants per plate.
>=20 Does anyone have any suggestions as to how I can keep my plants
>=20 healthy and thriving under this system? As well, for large rosettes, ho=
>=20 long can I keep the plants growing?
>=20 Wendy Allan,
Jim Tokuhisa, Ph.D.
Max Planck Institut f=FCr Chemische =D6kologie
E-mail: tokuhisa at ice.mpg.de
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