Number of t-DNA insertions?
cas3s at virginia.edu
Wed Sep 5 13:50:07 EST 2001
The original paper describing vacuum infiltration addressed
this - by Bechtold et al 1993, C.R. Acad Sci. Paris,
316:1194. They found 70% tandem copy insertions, 30% single
copy, and 63% 1 locus insertions, 21% 2 loci.
--On Wednesday, September 05, 2001 10:26 AM +0100 John
Runions <cjr41 at cam.ac.uk> wrote:
> Hi all,
> I don't think I dreamed that I saw a paper describing the
> average number of t-dna insertions in Arabidopsis
> following Agrobacterium-mediated transformation but I
> can't find it anywhere. Approximately 8 months ago I saw
> the paper while casually browsing the literature in the
> library but I didn't make note of the reference.
> Literature searches have not turned it up using either
> the keywords 't-dna' 'agrobacteri*' 'transformation' etc.
> Please tell me I'm not crazy? Does anyone know of the
> paper or does anyone have numbers that they would be
> willing to share?
> On the other hand though, I am very much more up on the
> literature than I was a few days ago. (<- Poetry).
> BTW the paper I'm looking for isn't: Young et al. (2001)
> Efficient Screening of Arabidopsis T-DNA Insertion Lines
> Using Degenerate Primers. Plant Physiol. 2001 125 (2):
> p. 513.
> Thanks for your help, John.
> C. John Runions, Ph. D.
> Department of Plant Sciences
> University of Cambridge
> Downing St.
> UK CB2 3EA
> email: runions at plantsci.cam.ac.uk
> phone: (01223) 766 545
Carla Green Lab
University of Virginia
Department of Biology
NSF Center For Biological Timing
Charlottesville, VA 22903
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