Number of t-DNA insertions?

Carl Strayer cas3s at virginia.edu
Wed Sep 5 13:50:07 EST 2001


<x-flowed>Hi John,

The original paper describing vacuum infiltration addressed
this - by Bechtold et al 1993, C.R. Acad Sci. Paris,
316:1194. They found 70% tandem copy insertions, 30% single
copy, and 63% 1 locus insertions, 21% 2 loci.

Carl

--On Wednesday, September 05, 2001 10:26 AM +0100 John
Runions <cjr41 at cam.ac.uk> wrote:

>  Hi all,
>
>  I don't think I dreamed that I saw a paper describing the
>  average number of t-dna insertions in Arabidopsis
>  following Agrobacterium-mediated transformation but I
>  can't find it anywhere.  Approximately 8 months ago I saw
>  the paper while casually browsing the literature in the
>  library but I didn't make note of the reference.
>  Literature searches have not turned it up using either
>  the keywords 't-dna' 'agrobacteri*' 'transformation' etc.
>  Please tell me I'm not crazy?  Does anyone know of the
>  paper or does anyone have numbers that they would be
>  willing to share?
>
>  On the other hand though, I am very much more up on the
>  literature than I was a few days ago. (<- Poetry).
>
>  BTW the paper I'm looking for isn't: Young et al. (2001)
>  Efficient Screening of Arabidopsis T-DNA Insertion Lines
>  Using Degenerate Primers.   Plant Physiol. 2001 125 (2):
>  p. 513.
>
>  Thanks for your help, John.
>
>  --
>  C. John Runions, Ph. D.
>  Department of Plant Sciences
>  University of Cambridge
>  Downing St.
>  Cambridge
>  UK   CB2 3EA
>
>  email: runions at plantsci.cam.ac.uk
>  phone: (01223) 766 545
>
>  http://www.plantsci.cam.ac.uk/Haseloff/JohnRunions/Home.h
>  tml
>
>
>  ---



---------------------------------
Carl Strayer

Carla Green Lab
University of Virginia
Department of Biology
NSF Center For Biological Timing
Gilmer Hall
Charlottesville, VA  22903
ph:434-243-2541 fax:434-982-5626
---------------------------------

---

</x-flowed>




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