yeast cDNA library strategy

Nobody nobody at
Wed Feb 6 11:47:19 EST 2002


I am currently constructing a yeast cDNA library ( 3 Reading frame vector,
directed cloning SSTI/NotI, fusion protein-cDNA construct). I would like to
know the number of clones you need to obtain after bacterial
transformation (primary clones), before making the transformation of the
library in yeast.
=46or the ligation of cDNA into the vector, what are the important steps to =
for defining the optimal ratio of cDNA / vector ?
What is the strategy after transfection in Bacteria (plating/cDNA library
preparation) ?
Thank you very much in advance,

Helene Storez
storez at



More information about the Arab-gen mailing list