[Arabidopsis] miRNA

R. Erdmann via arab-gen%40net.bio.net (by robert.erdmann At uni-bremen.de)
Thu Dec 14 03:47:31 EST 2006



has anybody of you experience with amiRNA controls? What is the state of art? 


Referring to siRNA-Experiments: If you are not able to conduct a knock-in of your target gene, you would design at least three siRNAs and negative controls (e.g. scrambled RNA or siRNA with no target gene) to proof the specific knock-down of the gene.




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