Did you sequence the mutation? Have you considered the possibility of
aberrant splicing?
Amy
University of Illinois
On May 5, 5:36 pm, xinyou gao <gaoxiny... from yahoo.com> wrote:
> Dear all,
> I am working on A gene in Arabidopsis thaliana. The A gene mutated plant is much short than WT plants, then transformed the A gene into the plants with 2 constructs:
> Construct 1 = 35S promoter + A gene cDNA sequence,
> Construct 2 = A gene promoter + A gene genomic DNA sequence
>> The phenotype was completely rescued by construct 2 transformation, but only partially rescued by construct 1 transformation.
>> I think the 35S promometr can not make A gene protein in the right places or enough amount.
>> Are they any other possibilities?
> Please email me the other possibilities if you think it may have!
> Thank you very much!
> Gao
> California, USA
>> ---------------------------------
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