IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

[Arabidopsis] Re: 35S promoter and native promoter

asdunlap from gmail.com via arab-gen%40net.bio.net (by asdunlap from gmail.com)
Tue May 8 09:14:36 EST 2007

Did you sequence the mutation?  Have you considered the possibility of
aberrant splicing?

University of Illinois

On May 5, 5:36 pm, xinyou gao <gaoxiny... from yahoo.com> wrote:
> Dear all,
>   I am working on A gene in Arabidopsis thaliana. The A gene mutated plant is much short than WT plants, then transformed the A gene into the plants with 2 constructs:
>   Construct 1 = 35S promoter + A gene cDNA sequence,
>   Construct 2 = A gene promoter + A gene genomic DNA sequence
>   The phenotype was completely rescued by construct 2 transformation, but only partially rescued by construct 1 transformation.
>   I think the 35S promometr can not make A gene protein in the right places or enough amount.
>   Are they any other possibilities?
>   Please email me the other possibilities if you think it may have!
>   Thank you very much!
>   Gao
>   California, USA
> ---------------------------------
> Expecting? Get great news right away with email Auto-Check.
> Try the Yahoo! Mail Beta.

More information about the Arab-gen mailing list

Send comments to us at biosci-help [At] net.bio.net