[Arabidopsis] Expression of a fusionprotein

[Madlen Schiller]

Hello,

at the moment I try to verify a fusion protein in Arabidopsis. I have  
cloned the gen by using Gateway cloning (Invitrogen). I know that the  
DNA-sequence is right and integrated in the Arabidopsis genom. But  
unfortunately I get not transkripts (no RNA or Protein) in the  
T1-generation.
Does anyone has/had also trouble with the expression of fusion  
proteins. I read that it is possible that the transcript will be done  
in further generations? Or is it possible the the Gateway cloning  
system decreased the expression?

Thanks a lot,
Madlen