[Arabidopsis] BASTA resistance gene silencing

[Gabriel León]

Dear Colleagues,
 
   Does anybody experienced BASTA resistance gene silencing? We are screening a large collection of T-DNA knockouts (mainly SAIL and SM lines), and for some lines no resistant seedlings were rescued, even after sowing 70 seeds (all germinated, the phenotype is compatible with BASTA sensitive seedlings). This could be produced by a lethal mutation that segregates in a distorted way, but we are wondering if anybody has experienced BASTA gene silencing, as described for kanamycing in some Salk lines.
 
Thank you,
 
Gabriel
 
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Dr. Gabriel León
Profesor Asistente
Centro de Biotecnología Vegetal
Universidad Andrés Bello
República 217, 1 piso
Santiago - CHILE
Tel: +56-2-7703035
Fax: +56-2-6615832
www.unab.cl