[Arabidopsis] Re: Arab-gen Digest, Vol 51, Issue 7

[Cesar Barragan]

Mr, Abo-Ogiala

You were targeting seeds for segregated lines.  Do you have any PCR  
pictures we could see?  How did you genotype these lines?
A homozygous line has being already isolated for gene At5g02020, you  
can request SALK_064028C to ABRC.
Thanks

Cesar Barragan
The Salk Institute for Biological Studies
Genomic Analysis Laboratory
10010 North Torrey Pines Road
La Jolla, CA 92037
Tel 858-453-4100 ext 1108
barragan from salk.edu


On Jul 15, 2009, at 11:11 AM, Ronan OMalley wrote:

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> From:  <arab-gen-request from oat.bio.indiana.edu>
> Date: Wed, Jul 15, 2009 at 9:03 AM
> Subject: Arab-gen Digest, Vol 51, Issue 7
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>   1. At5g02020 (Abo-Ogiala, Atef)
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> Message: 1
> Date: Tue, 14 Jul 2009 11:14:26 +0200
> From: "Abo-Ogiala, Atef" <Atef.Abo-Ogiala from forst.uni-goettingen.de>
> Subject: [Arabidopsis] At5g02020
> To: <arab-gen from magpie.bio.indiana.edu>
> Message-ID:
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>
> Hello,
>
> We ordered SALK_040413 of the gene At5g02020. We have problem with  
> this line.
> The whole plants that we get from the seeds are wild type. Please  
> if you have
> homozygous line for At5g02020 gene except Salk_146631 tell me from  
> which
> source I can get it.
>
> Best regards
>
> Atef Abo-Ogiala
>
> PhD student
>
> Büsgen-Institut
>
> Forstbotanik und Baumphysiologie
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> Georg-August Universität
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> 37077 Goettingen
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> Germany
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> Tel:  +49 (0)551 - 39 9362
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