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[Arabidopsis] AW: mRNA and sRNA from seedlings (Slotkin, R. Keith)

Domenica Hamisch via arab-gen%40net.bio.net (by d.hamisch from tu-bs.de)
Wed Feb 22 03:13:29 EST 2012

Hello Mr. Slotkin,

perhaps it is better to use the dynabeads kit- we used it for mRNA
preparation- but in a slightly modificated way- and it was OK- mRNA quality
was OK as well (checked with Bioanlyzer). Maybe this is an option to think

Best regards,

Domenica Hamisch (MSc Biologie)
AG Mendel
Institut für Pflanzenbiologie
Technische Universität Braunschweig
Humboldtstraße 1
38106 Braunschweig

Tel.: 0531/391-5875
E-Mail: d.hamisch from tu-bs.de

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Today's Topics:

   1. mRNA and sRNA from seedlings (Slotkin, R. Keith)


Message: 1
Date: Mon, 20 Feb 2012 11:09:25 +0000
From: "Slotkin, R. Keith" <slotkin.2 from osu.edu>
Subject: [Arabidopsis] mRNA and sRNA from seedlings
To: "arab-gen from net.bio.net" <arab-gen from magpie.bio.indiana.edu>
Message-ID: <C2E34BB5-C5A5-4070-923A-63E824313D84 from osu.edu>
Content-Type: text/plain; charset="us-ascii"


My lab has noticed a very poor efficiency for isolating both mRNA and small
RNA from some tissues, while other tissues seem fine. We are using
Invitrogen's (now Life Technologies) Trizol product. As far as we can tell,
there are abundant mRNA and sRNA before the isolation, but the Trizol seems
to select for long non-mRNA. Invitrogen says the tissue shouldn't matter,
but experimentally we see that it does. Can anyone suggest an RNA prep that
can work from ANY tissue (seedling, seed, etc...), isolate all small RNAs
(20-25nt), and recover a high percentage of the longer mRNAs?

Thank you,

R. Keith Slotkin
Assistant Professor
The Ohio State University

500 Aronoff Laboratory
318 West 12th Ave.
Columbus, OH, 43210

slotkin.2 from osu.edu<mailto:slotkin.2 from osu.edu>


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