[Arabidopsis] Re: Sequence verified Col-0 ecotype

Slotkin, Keith via arab-gen%40net.bio.net (by slotkin.2 from osu.edu)
Wed Jun 14 20:50:11 EST 2017


Dear Vjay and the Arabidopsis community,

The ABRC has many different “wild-type Columbia” stocks. The sequenced reference genome strain is CS70000. This is the progeny line of the wt Col line used for Salk T-DNA insertions. 

http://www.arabidopsis.org/servlet/TairObject?id=3501642308&type=stock

If you are planning a genome-wide based analysis, I highly suggest you replace your laboratory stocks with this reference line, as we have detected polymorphisms between lab curated “wt Col-0” strains and the reference genome. 

Best,

R. Keith Slotkin
Associate Professor
Department of Molecular Genetics
The Ohio State University

Co-Director of the Arabidopsis Biological Resource Center (ABRC)
www.abrc.osu.edu

500 Aronoff Laboratory
318 West 12th Ave.
Columbus, OH, 43210

slotkin.2 from osu.edu
http://molgen.osu.edu/people/slotkin.2
614-292-1087


On Jun 12, 2017, at 1:03 PM, arab-gen-request from oat.bio.indiana.edu wrote:

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Today's Topics:

  1. Sequence verified Col-0 ecotype (Vijaykumar Veerappan)


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Message: 1
Date: Thu, 8 Jun 2017 22:15:14 -0400
From: Vijaykumar Veerappan <vijaymull from gmail.com>
Subject: [Arabidopsis] Sequence verified Col-0 ecotype
To: arab-gen from magpie.bio.indiana.edu
Message-ID:
	<CAHvD93osOOF8cf7oBczzS5GudX9UaUt7LkBs8CGKU3n9GFrsEQ from mail.gmail.com>
Content-Type: text/plain; charset="UTF-8"

Hello,

I need to order Col-0 wild-type seeds (sequence verified for purity). From
where do I order (ABRC or other sources) and please give me the stock
number.
Last time I ordered was in 2004 from ABRC. I remember the stock number
was CS60000. I'm not sure it is still the same.



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