[Automated-sequencing] ALf express

[Sabine Lautenschlaeger]

Hi,

 

I’m also sequencing PCR-Products using Alf Express.

 

I clean the PCR-Product from the old primers using the “PCR-kit” from GE. 

This product is used directly without any ss-ends.

Instead of cy5-dATP I have sequencing primers 5’labeled with cy5.

The sequencing primer are positioned like a “nested PCR-primer” a little bit
away from the end of the PCR product.

The sequencing reaction is carried out using the “Sequencing-Kit” from GE.

If you are interested in the protokoll, please let me know.

 

Saluti and ciao

 

Sabine Lautenschläger