[Automated-sequencing] ALf express
(by s.lautenschlaeger from labor-brunner.de)
Tue Nov 11 09:11:00 EST 2008
Im also sequencing PCR-Products using Alf Express.
I clean the PCR-Product from the old primers using the PCR-kit from GE.
This product is used directly without any ss-ends.
Instead of cy5-dATP I have sequencing primers 5labeled with cy5.
The sequencing primer are positioned like a nested PCR-primer a little bit
away from the end of the PCR product.
The sequencing reaction is carried out using the Sequencing-Kit from GE.
If you are interested in the protokoll, please let me know.
Saluti and ciao
More information about the Autoseq