[Automated-sequencing] T3 and T7 primers

[Buffet, Eleonore]

Dear Kenneth,

                            Hi I'm a student having a problem with some
extra research I am conducting. I have come across numerous definitions
as to the function(s) of T7 and T3. Could you please provide your expert
opinion as to which are false? 

To transfer a plasmid to other bacteria 

Used as a priming site (my personal preference)

Can be used to make ssRNA copies of DNA

A promoter for cloned DNA expression

Viral RNApol promoter

 

Again, many thanks for you time and excellent postings online. 

 

Wishing you much Happy sequencing!

Love Eleonore xx