[Automated-sequencing] TET-labeled markers on a ABI 3130xl

J. Albert Vallunen via autoseq%40net.bio.net (by albval from utu.fi)
Tue Oct 21 06:57:44 EST 2008


Hi all,

We recently obtained a large quantity of microsatellite markers that we 
would need to screen as soon as possible. The problem is that the dye 
set of the primers is FAM, HEX and TET and not the usual three-colour 
triplet FAM, HEX and NED that works fine with the 3130.

Since getting nearly a hundred of new NED-labeled primers to replace the 
TET-ones really isn't an option I was wondering if anyone knows a way to 
run TET-labeled markers on an ABI3130? Or is it impossible? I gather we 
would need at least a TET-containing spectral calibration kit / matrix 
for the procedure to succeed.

I know already that the "flourescent amidite matrix standard kit" for 
the 310 system which is found on the ABI web page has the correct 
labels, but is incompatible with 3130 due to having multiple peaks. 
There also doesn't seem to be any other ready-to-use standards to meet 
the criteria. I've asked also the local ABI tech support for an answer, 
but they're still yet to provide a working solution.

Any help on the matter would be greatly appreciated,

Yours,

Albert Vallunen

-- 
J. Albert Vallunen, M.Sc., Graduate student
Laboratory of Genetics, Dept. of Biology
20014 University of Turku, Finland
tel. +358 2 333 7085

Home address:
Valaskalliontie 85
23140 Hietamäki, Finland
tel. +358 40 562 8587



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