From owner-contents@net.bio.net Mon Mar 02 22:00:00 1998 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.journals.contents Subject: Blood Cells, Molecules, & Diseases, vol. 24, no. 04, 28 February 1998 Date: 2 Mar 1998 16:03:01 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 90 Sender: daemon@net.bio.net Approved: biosci-help@net.bio.net Distribution: world Message-ID: <6dfhbl$amg@net.bio.net> NNTP-Posting-Host: net.bio.net AU Kanno-H. Fujii-H. Miwa-S. TI Expression and Enzymatic Characterization of Human Glucose Phosphate Isomerase (GPI) Variants Accounting for GPI Deficiency SO Blood-Cells-Mol-Dis. 1998 Feb 28. 24(4). P. 54-61. DE hemolytic anemia DE erythroenzymopathy DE glycolysis DE point mutation DE amino acid substitution AB To elucidate the structure-function relationships in glucose phosphate isomerase (GPI), we established an expression system for human GPI as a fusion protein with glutathione S-transferase (GST) in E. coli. The GST-GPI fusion protein showed affinities for the substrates glucose 6-phosphate (G6P) and fructose 6-phosphate (F6P) similar to those of the native enzyme purified from human red blood cells (RBC). We expressed GPI cDNAs with four distinct disease-causing mutations and examined their enzymatic characteristics. Although each mutation caused reduced thermal stability, an amino acid substitution Thr-5->Ile (T5I) exhibited marked thermal instability, suggesting that the amino-terminal of GPI is important for enzymatic stability. Thr-224 seemed not to be an essential residue, since the amino acid substitution Thr-224->Met (T224M) showed normal substrate affinity in spite of a slight decrease in both specific activity and thermostability. Gln-343 and Asp-539 have been shown to be in close proximity to the putative catalytic sites, and the present study showed that both Gln-343->Arg (Q343R) and Asp-539->Asn (D539N) caused impaired substrate affinity; Q343R showed high Km for both G6P and F6P, whereas D539N showed significantly decreased affinity only for F6P. These results suggest that not only reduced enzymatic stability but also impaired kinetics may disturb RBC metabolism of the GPI variants associated with hereditary hemolytic anemia. CC Reprint request to: Hitoshi Kanno, M.D., Ph.D., Okinaka CC Memorial Institute for Medical Research, 2-2-2 Toranomon, CC Minato-ku, Tokyo 105, Japan, phone 81-3-3588-1111 Ext 4415, CC fax 81-3-3505-6205, email: hikanno@med.email.ne.jp AU Segel-M.J. Paltiel-O. Zimran-A. Gottschalk-Sabag-S. Schibi-G. Krichevski-S. Ludkovski-O. Yehuda-D.B. TI Geographic Variance in the Frequency of the t(14;18) Translocation in Follicular Lymphoma: An Israeli Series Compared to the World Literature SO Blood-Cells-Mol-Dis. 1998 Feb 28. 24(4). P. 62-72. DE follicular lymphoma DE epidemiology DE t(14;18) DE Israel DE Europe DE Asia DE United States AB It has been suggested that differences in the frequency of the t(14;18) translocation in follicular lymphoma might explain ethno-geographic variation in the incidence of these tumors. We tested Israeli follicular lymphoma patients for the frequency of the t(14;18) translocation, and reviewed the published literature, comparing the frequency in our series with data from different parts of the world. Tissue specimens from 36 Israeli follicular lymphoma patients were tested for presence of the translocation by PCR amplification of the MBR breakpoint. Twenty-two of the 36 patients (61%) tested positive. A systematic search of the literature yielded 35 papers reporting the frequency of the t(14;18) translocation in follicular lymphoma. We analyzed cytogenetic data and molecular data separately. For each method, data were pooled from all studies within each of three geographical regions - USA, East Asia and Europe. Pooled data from cytogenetic studies show a low frequency of the translocation in the Far East (38%) compared to the USA (71%), with an intermediate frequency found in Europe (61%). Molecular studies show a similar frequency of the translocation in the Far East and Europe, significantly lower than the frequency in pooled data from American studies. The frequency in our Israeli series is relatively high, comparable to that detected in the USA. We suggest that the apparent geographical differences we describe are unlikely to be caused by a difference in the biology of the tumor, and are more likely due to technical and methodological factors. We conclude that it is unlikely that differences in the frequency of the t(14;18) translocation explain the difference in the epidemiology of lymphoma between East and West. CC Reprint request to: Dr. Dina Ben Yehuda, Department of CC Hematology, Hadassah University Hospital, Kiryat Hadassah POB CC 12000, Jerusalem, Israel, phone 672-2-6776744, fax 672-2-6423067, CC email: dbyehuda@hadassah.org.il From owner-contents@net.bio.net Fri Mar 06 22:00:00 1998 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.journals.contents Subject: Marine Ecology Progress Series, vol. 163, no. x, 12 March 1998 Date: 6 Mar 1998 21:16:42 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 197 Sender: daemon@net.bio.net Approved: biosci-help@net.bio.net Distribution: world Message-ID: <6dql7q$a01@net.bio.net> NNTP-Posting-Host: net.bio.net CC Inter-Research Science Publisher CC Internet Service CC MEPS 163 CC Table of contents CC ------------------------------------------------------------------- CC Abstracts from Inter-Research journals, along with a searchable CC index, are available on the Internet (http://www.int-res.com). CC Contents are also sent to a mailing list (contents@int-res.com). CC Inter-Research, Nordbuente 23, D-21385 Oldendorf/Luhe, Germany CC Tel: (+49)(0) 4132 7127 CC Fax: (+49)(0) 4132 8883 CC E-mail: ir@int-res.com CC Copyright Inter-Research, Oldendorf/Luhe, 1998 CC ------------------------------------------------------------------- CC ------------------------------------------------------------------- CC Marine Ecology Progress Series (ISSN 0171-8630) CC Volume 163 (1998) CC March 12 CC ------------------------------------------------------------------- CC RESEARCH ARTICLES AU Lawson-J-W. Anderson-J-T. Dalley-E-L. Stenson-G-B. TI Selective foraging by harp seals Phoca groenlandica in nearshore and offshore waters of Newfoundland, 1993 and 1994. SO Mar-Ecol-Prog-Ser. 1998 Mar 12. 163. P 1-10. AU Brodeur-R-D. TI In situ observations of the association between juvenile fishes and scyphomedusae in the Bering Sea. SO Mar-Ecol-Prog-Ser. 1998 Mar 12. 163. P 11-20. AU Vance-D-J. Haywood-M-D-E. Heales-D-S. Kenyon-R-A. Loneragan-N-R. TI Seasonal and annual variation in abundance of postlarval and juvenile banana prawns Penaeus merguiensis and environmental variation in two estuaries in tropical northeastern Australia: a six year study. SO Mar-Ecol-Prog-Ser. 1998 Mar 12. 163. P 21-36. AU Uye-S. Sano-K. TI Seasonal variations in biomass, growth rate and production rate of the small cyclopoid copepod Oithona davisae in a temperate eutrophic inlet. SO Mar-Ecol-Prog-Ser. 1998 Mar 12. 163. P 37-44. AU Curran-M-C. Able-K-W. TI The value of tethering fishes (winter flounder and tautog) as a tool for assessing predation rates. SO Mar-Ecol-Prog-Ser. 1998 Mar 12. 163. P 45-51. AU Guisande-C. Riveiro-I. Sola-A. Valdes-L. TI Effect of biotic and abiotic factors on the biochemical composition of wild eggs and larvae of several fish species. SO Mar-Ecol-Prog-Ser. 1998 Mar 12. 163. P 53-61. AU Mari-X. Burd-A. TI Seasonal size spectra of transparent exopolymeric particles (TEP) in a coastal sea and comparison with those predicted using coagulation theory. SO Mar-Ecol-Prog-Ser. 1998 Mar 12. 163. P 63-76. AU Schumann-R. Rentsch-D. TI Staining particulate organic matter with DTAF--a fluorescence dye for carbohydrates and protein: a new approach and application of a 2D image analysis system. SO Mar-Ecol-Prog-Ser. 1998 Mar 12. 163. P 77-88. AU Kinney-E-H. Roman-C-T. TI Response of primary producers to nutrient enrichment in a shallow estuary. SO Mar-Ecol-Prog-Ser. 1998 Mar 12. 163. P 89-98. AU Gilbert-F. Stora-G. Bonin-P. TI Influence of bioturbation on denitrification activity in Mediterranean coastal sediments: an in situ experimental approach. SO Mar-Ecol-Prog-Ser. 1998 Mar 12. 163. P 99-107. AU Trimmer-M. Nedwell-D-B. Sivyer-D-B. Malcolm-S-J. TI Nitrogen fluxes through the lower estuary of the river Great Ouse, England: the role of the bottom sediments. SO Mar-Ecol-Prog-Ser. 1998 Mar 12. 163. P 109-124. AU Vedel-A. TI Phytoplankton depletion in the benthic boundary layer caused by suspension-feeding Nereis diversicolor (Polychaeta): grazing impact and effect of temperature. SO Mar-Ecol-Prog-Ser. 1998 Mar 12. 163. P 125-132. AU Katajisto-T. Viitasalo-M. Koski-M. TI Seasonal occurrence and hatching of calanoid eggs in sediments of the northern Baltic Sea. SO Mar-Ecol-Prog-Ser. 1998 Mar 12. 163. P 133-143. AU Aarnio-K. Bonsdorff-E. Norkko-A. TI Role of Halicryptus spinulosus (Priapulida) in structuring meiofauna and settling macrofauna. SO Mar-Ecol-Prog-Ser. 1998 Mar 12. 163. P 145-153. AU De-Wolf-H. Backeljau-T. Verhagen-R. TI Spatio-temporal genetic structure and gene flow between two distinct shell morphs of the planktonic developing periwinkle Littorina striata (Mollusca: Prosobranchia). SO Mar-Ecol-Prog-Ser. 1998 Mar 12. 163. P 155-163. AU Comtet-T. Desbruyeres-D. TI Population structure and recruitment in mytilid bivalves from the Lucky Strike and Menez Gwen hydrothermal vent fields (37*17'N and 37*50'N on the Mid-Atlantic Ridge). SO Mar-Ecol-Prog-Ser. 1998 Mar 12. 163. P 165-177. AU Abele-D. Grosspietsch-H. Poertner-H-O. TI Temporal fluctuations and spatial gradients of environmental PO2, temperature, H2O2 and H2S in its intertidal habitat trigger enzymatic antioxidant protection in the capitellid worm Heteromastus filiformis. SO Mar-Ecol-Prog-Ser. 1998 Mar 12. 163. P 179-191. AU Stien-A. Leinaas-H-P. Halvorsen-O. Christie-H. TI Population dynamics of the Echinomermella matsi (Nematoda)-Strongylocentrotus droebachiensis (Echinoida) system: effects on host fecundity. SO Mar-Ecol-Prog-Ser. 1998 Mar 12. 163. P 193-201. AU Benedetti-Cecchi-L. Bulleri-F. Cinelli-F. TI Density dependent foraging of sea urchins in shallow subtidal reefs on the west coast of Italy (western Mediterranean). SO Mar-Ecol-Prog-Ser. 1998 Mar 12. 163. P 203-211. AU Roberts-D-E. Smith-A. Ajani-P. Davis-A-R. TI Rapid changes in encrusting marine assemblages exposed to anthropogenic point-source pollution: a 'Beyond BACI' approach. SO Mar-Ecol-Prog-Ser. 1998 Mar 12. 163. P 213-224. AU Miller-K-J. TI Short-distance dispersal of black coral larvae: inference from spatial analysis of colony genotypes. SO Mar-Ecol-Prog-Ser. 1998 Mar 12. 163. P 225-233. AU Ben-David-Zaslow-R. Benayahu-Y. TI Competence and longevity in planulae of several species of soft corals. SO Mar-Ecol-Prog-Ser. 1998 Mar 12. 163. P 235-243. AU Ricciardi-A. Bourget-E. TI Weight-to-weight conversion factors for marine benthic macroinvertebrates. SO Mar-Ecol-Prog-Ser. 1998 Mar 12. 163. P 245-251. AU Plante-C-J. Shriver-A-G. TI Patterns of differential digestion of bacteria in deposit feeders: a test of resource partitioning. SO Mar-Ecol-Prog-Ser. 1998 Mar 12. 163. P 253-258. AU Bidwell-J-R. Wheeler-K-W. Burridge-T-R. TI Toxicant effects on the zoospore stage of the marine macroalga Ecklonia radiata (Phaeophyta: Laminariales). SO Mar-Ecol-Prog-Ser. 1998 Mar 12. 163. P 259-265. AU Dalla-Via-J. Sturmbauer-C. Schoenweger-G. Soetz-E. Mathekowitsch-S. Stifter-M. Rieger-R. TI Light gradients and meadow structure in Posidonia oceanica: ecomorphological and functional correlates. SO Mar-Ecol-Prog-Ser. 1998 Mar 12. 163. P 267-278. CC NOTES AU Nagelkerken-I. Bak-R-P-M. TI Differential regeneration of artificial lesions among sympatric morphs of the Caribbean corals Porites astreoides and Stephanocoenia michelinii. SO Mar-Ecol-Prog-Ser. 1998 Mar 12. 163. P 279-283. AU Takeuchi-I. Watanabe-K. TI Respiration rate and swimming speed of the necrophagous amphipod Eurythenes gryllus from Antarctic deep waters. SO Mar-Ecol-Prog-Ser. 1998 Mar 12. 163. P 285-288. AU Ceccherelli-G. Cinelli-F. TI Habitat effect on spatio-temporal variability in size and density of the introduced alga Caulerpa taxifolia. SO Mar-Ecol-Prog-Ser. 1998 Mar 12. 163. P 289-294. AU Wilson-R-P. Kierspel-M-A-M. TI A method for retrieval of anchored stomach probes from seabirds. SO Mar-Ecol-Prog-Ser. 1998 Mar 12. 163. P 295-297. CC COMMENTS AU Kaehler-P. TI Concerning respiration and the carbon-to-nitrogen ratio of a phytoplankton bloom. Comment on Kepkay et al. (1997). SO Mar-Ecol-Prog-Ser. 1998 Mar 12. 163. P 299-301. AU Kepkay-P-E. TI Reply to the comment of P. Kaehler. SO Mar-Ecol-Prog-Ser. 1998 Mar 12. 163. P 303-305. From owner-contents@net.bio.net Fri Mar 06 22:00:00 1998 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.journals.contents Subject: Diseases of Aquatic Organisms, vol. 32, no. 02, 5 March 1998 Date: 6 Mar 1998 21:13:43 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 86 Sender: daemon@net.bio.net Approved: biosci-help@net.bio.net Distribution: world Message-ID: <6dql27$9m8@net.bio.net> NNTP-Posting-Host: net.bio.net CC Inter-Research Science Publisher CC Internet Service CC DAO 32(2) CC Table of contents CC ------------------------------------------------------------------- CC Abstracts from Inter-Research journals, along with a searchable CC index, are available on the Internet (http://www.int-res.com). CC Contents are also sent to a mailing list (contents@int-res.com). CC Inter-Research, Nordbuente 23, D-21385 Oldendorf/Luhe, Germany CC Tel: (+49)(0) 4132 7127 CC Fax: (+49)(0) 4132 8883 CC E-mail: ir@int-res.com CC Copyright Inter-Research, Oldendorf/Luhe, 1998 CC ------------------------------------------------------------------- CC ------------------------------------------------------------------- CC Diseases of Aquatic Organisms (ISSN 0177-5103) CC Volume 32, Number 2 (1998) CC March 5 CC ------------------------------------------------------------------- CC RESEARCH ARTICLES AU Supamattaya-K. Hoffmann-R-W. Boonyaratpalin-S. Kanchanaphum-P. TI Experimental transmission of white spot syndrome virus (WSSV) from black tiger shrimp Penaeus monodon to the sand crab Portunus pelagicus, mud crab Scylla serrata and krill Acetes sp. SO Dis-Aquat-Org. 1998 Mar 5. 32(2). P 79-85. AU Oshima-S. Hata-J-I. Hirasawa-N. Ohtaka-T. Hirono-I. Aoki-T. Yamashita-S. TI Rapid diagnosis of red sea bream iridovirus infection using the polymerase chain reaction. SO Dis-Aquat-Org. 1998 Mar 5. 32(2). P 87-90. AU Midtlyng-P-J. Lillehaug-A. TI Growth of Atlantic salmon Salmo salar after intraperitoneal administration of vaccines containing adjuvants. SO Dis-Aquat-Org. 1998 Mar 5. 32(2). P 91-97. AU Chen-S-C. Adams-A. Thompson-K-D. Richards-R-H. TI Electron microscope studies of the in vitro phagocytosis of Mycobacterium spp. by rainbow trout Oncorhynchus mykiss head kidney macrophages. SO Dis-Aquat-Org. 1998 Mar 5. 32(2). P 99-110. AU Lopez-Doriga-M-V. Martinez-J-L. TI Ultrastructure of fish cells involved in cellular defences against Saprolegnia infections: evidence of non-leucocytic nature. SO Dis-Aquat-Org. 1998 Mar 5. 32(2). P 111-117. AU Stehr-C-M. Johnson-L-L. Myers-M-S. TI Hydropic vacuolation in the liver of three species of fish from the U.S. West Coast: lesion description and risk assessment associated with contaminant exposure. SO Dis-Aquat-Org. 1998 Mar 5. 32(2). P 119-135. AU Wuertz-J. Knopf-K. Taraschewski-H. TI Distribution and prevalence of Anguillicola crassus (Nematoda) in eels Anguilla anguilla of the rivers Rhine and Naab, Germany. SO Dis-Aquat-Org. 1998 Mar 5. 32(2). P 137-143. AU Canestri-Trotti-G. Baccarani-E-M. Giannetto-S. Giuffrida-A. Paesanti-F. TI Prevalence of Mytilicola intestinalis (Copepoda: Mytilicolidae) and Urastoma cyprinae (Turbellaria: Hypotrichinidae) in marketable mussels Mytilus galloprovincialis in Italy. SO Dis-Aquat-Org. 1998 Mar 5. 32(2). P 145-149. CC NOTES AU Robertson-P-A-W. Calderon-J. Carrera-L. Stark-J-R. Zherdmant-M. Austin-B. TI Experimental Vibrio harveyi infections in Penaeus vannamei larvae. SO Dis-Aquat-Org. 1998 Mar 5. 32(2). P 151-155. AU Moravec-F. Nagasawa-K. Tanaka-Y. Narita-T. TI Role of Leptodora kindti (Cladocera: Leptodoridae) in the life cycle of Raphidascaris biwakoensis (Nematoda: Anisakidae), a fish parasite in Lake Biwa, Japan. SO Dis-Aquat-Org. 1998 Mar 5. 32(2). P 157-160. From owner-contents@net.bio.net Sun Mar 15 22:00:00 1998 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.journals.contents Subject: Biotecnologia Aplicada, vol. 15, no. 01, 1998 Date: 15 Mar 1998 22:05:24 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 314 Sender: daemon@net.bio.net Approved: biosci-help@net.bio.net Distribution: world Message-ID: <6eiff4$1h6@net.bio.net> NNTP-Posting-Host: net.bio.net BIOTECNOLOGIA APLICADA CC Vol. 15 No. 1 (January-March, 1998) CC ISSN 0864-4551 (printed) CC ISSN 1027-2852 (electronic) CC Copyright 1998, Elfos Scientiae. CC Founded in 1983 and published as Interfer=F3n y CC Biotecnolog=EDa until 1990, Biotecnolog=EDa Aplicada is a CC peer-reviewed scientific journal. It appears four times CC per year, sponsored by the Ibero-Latin-American Society CC of Biotechnology Applied to Health. CC BIOTECNOLOGIA APLICADA is a vehicle of Iberian-Latin- CC American scientists working in different fields of modern CC biotechnology for the diffusion of their experiences and CC results. Some of the main topics that are considered for CC publication are the following: CC a) Molecular genetics and recombinant DNA technology; CC b) Therapeutic and prophylactic uses of molecules CC obtained by biotechnological methods; CC c) Immunotechnology and development of diagnostic CC methods; CC d) Chemistry, biochemistry and design of molecules with CC biological activity; CC e) Application of recombinant DNA techniques and its CC products in agriculture and animal science; CC f) Transgenesis; CC g) Fermentation, recovery and purification of biotech- CC nological products; CC h) Scaling-up, plant design and economic evaluation; CC i) Control and automation of processes. CC All rights reserved. No part of this publication may be CC reproduced, stored or transmitted in any form or by any CC means without the prior permission of the Publisher. By CC submitting a manuscript the authors agree that the CC copyright for their article is transferred to the CC Publisher if and when the article is accepted for CC publication. The points of view expressed in the Journal CC are exclusive responsibility of the authors. CC This journal is indexed by the following publications: CC Biosis, Current Biotechnology Abstracts, Derwent Biotech- CC nology Abstracts, Cambridge Scientific Abstracts, CC Chemical Abstracts, Biological Abstracts, Index Medicus, CC LilaCS, Bio-Journals (Internet). CC Correspondence and subscriptions CC Ave. 31 entre 158 y 190, CC Cubanac=E1n, Playa CC Ciudad de La Habana, CC Apdo. 6072, Habana 6, Cuba. CC Telephones: (53-7) 21-8466 / 21-8164 CC Fax: (53-7) 21-8070 / 33-6008 CC E-mail: elfos@cigb.edu.cu CC Full papers are available online through BIOLINE System CC (Internet) through the WWW or by e-mail. Annual subscrip- CC tion or single document purchase are possible. Contact CC the following for further information: CC URL:http://www.bd.org.br/bioline/ CC E-mail address: bio@biostrat.demon.co.uk AU Dania V=E1zquez Blomquist IN Divisi=F3n de Vacunas, Centro de Ingenier=EDa Gen=E9tica y Biotecnolog=EDa, apartado postal 6162, La Habana 10600, Cuba. Fax: (53-7) 218070, 336008; E-mail: carlos.duarte@cigb.edu cu TI Los poxvirus como vectores vivos: una promesa en la vacunaci=F3n DE poxviruses DE vaccinia DE ALVAC DE NYVAC DE HIV SO Biotecnologia Aplicada 1998;15:1-14 AB Vaccination against smallpox was the beginning of the use of poxviruses as live vectors. The first works in the molecular biology of vaccinia appeared in the 80's. Since that date many poxviruses have been used for vaccination of animals against different infectious diseases. In many cases, the vaccination with recombinant poxviruses confered protection against diseases. Nevertheless, the complications and side effects associated with vaccinia virus immunization led to the search of new variants of poxviruses such as, attenuated vaccinia and avipoxviruses. The last ones, have a very restricted host range, so they do not replicate in mammals but the antigens can be expressed at early steps of the life circle and induce an immune response. NYVAC (derivative of vaccinia) and ALVAC (derivative of canary pox) are two of the attenuated recombinants. They have demonstrated their security in preclinical assays, so phase I trials in humans have already begun. Until now, ALVAC expressing antigens from the human immunodeficiency virus, is the immunogen with the best results in the induction of a CD8+ T cell response in immunized seronegative individuals. These recombinants have become in a promise for the fight against the acquired immunodeficiency syndrome. CC Review Article CC Language:Spanish AU Reynold Morales, Am=EDlcar Arenal, Rafael Pimentel, Irian Mendoza, Asterio Cruz, Rebeca Mart=EDnez, Fidel Herrera, Leopoldo T=E1panes, Mario P Estrada, Jos=E9 de la Fuente IN Divisi=F3n de Gen=E9tica de C=E9lulas de Mam=EDferos. Divisi=F3n de Control de la Calidad. Centro de Ingenier=EDa Gen=E9tica y Biotecnolog=EDa. Apartado postal 6162, Ciudad de La Habana, Cuba. Centro de Ingenier=EDa Gen=E9tica y Biotecnolog=EDa. Apartado postal 387, Camag=FCey-1, Cuba. Estaci=F3n Acuadique, La Habana, Cuba TI Caracterizaci=F3n del fondo gen=E9tico de la l=EDnea de supertilapias IG/03-F70. I. Caracteres morfom=E9tricos, mer=EDsticos y an=E1lisis de ADN DE tilapia DE biometry DE chromosome DE DNA satellite SO Biotecnologia Aplicada 1998;15:15-21 AB Morphological, meristic and DNA differences have been established in the characterization of various strains of tilapia (Oreochromis spp). The present study shows the characterization of the transgenic line of supertilapia IG/03-F70. Morphological and meristic data show a high correlation with the tilapia Oreochromis hornorum. The chromosomal number and DNA satellite analysis support the hypothesis that the parents of supertilapias IG/03-F70 progenitors are hybrids of an unknown origen with a high percentage of O. hornorum. CC Research Article CC Language:Spanish AU Fernando Fern=E1ndez, Marisela Su=E1rez, Wilfredo D=EDaz, Hayd=E9e Ger=F3nimo, Fernando Porrero, Celia Fern=E1ndez- Ortega IN Banco de Sangre Provincial "Renato Guitart". Apartado postal 1040, Santiago de Cuba, Cuba. Centro de Investigaciones Biol=F3gicas. Apartado postal 6996, Ciudad de La Habana, Cuba. Telf: (53-7) 21 7465; Fax: (53-7) 21 0553; E-mail: prod@cib.cigb.edu.cu TI Efecto de la pasteurizaci=F3n sobre algunas propiedades del factor de transferencia DE pasteurization DE virucidal procedure DE transfer factor DE validation SO Biotecnologia Aplicada 1998;15:22-24 AB The influence of pasteurization on some physical- chemical and biological properties of the human transfer factor was studied. Results did not show significant changes in protein concentration, pH, UV spectrum and absorbance (260/280 nm) after the virucidal procedure. Leukocyte inhibitory factor stimulation was maintained after the pasteurization in all tested batches. CC Research Article CC Language:Spanish AU Eileen Riego, Ricardo Lleonart, Ma. Victoria Sa=EDnz de la Pe=F1a, Ferm=EDn Amaro, Ketty Bacallao, Marta Santiesteban, Felipe Rolo, Luis Herrera, Madelyn Blanco, Jos=E9 de la Fuente IN Centro de Ingenier=EDa Gen=E9tica y Biotecnolog=EDa. Apartado postal 6162, La Habana, Cuba. E-mail: orsup@cigb.edu.cu; Fax: (53-7) 21 8070 33 8008. Laboratorio Central de Criminal=EDstica. La Habana, Cuba. Instituto de Medicina Legal. La Habana, Cuba. Laboratorio de Investigaciones del SIDA, La Habana, Cuba TI Obtenci=F3n de ADN =FAtil para an=E1lisis de tipaje gen=E9tico a partir de restos =F3seos humanos antiguos DE genetic typing DE bone DE polymerase chain reaction DE PCR DE short tandem repeats DE STR DE mitochondrial DNA SO Biotecnologia Aplicada 1998;15:25-29 AB Although several methods have been described to extract human DNA from bone tissues, the results are variable and sometimes the purified DNA is not suitable for polymerase chain reaction analysis. Specially for forensic applications where PCR mediated typing of short tandem repeats STR loci is imperative, the presence of inhibitors of the T. aquaticus DNA polymerase is often an insoluble difficulty. Here we describe methods which has been used in our laboratory for some time already to do the genetic typing of several short tandem repeats loci on genomic DNA extracted from ancient bone remains as well as the amplification and sequencing of the hypervariable region of the human mitochondrial DNA from the same specimens. The usefulness of the method was documented even with bone remains 2 000 years old. These results are important not only from the forensic point of view but also for the applications of the so called "molecular archaeology techniques" to help trace back the origins of ancient human populations. CC Article on Techniques CC Language:Spanish AU Jos=E9 R Hern=E1ndez, Miriela Gil, Ya=ED Cruz, Rebeca Bouy=F3n, Jorge Segredo, Lorenzo Rod=E9s IN Divisi=F3n de Producci=F3n de Interferones; Divisi=F3n de Qu=EDmica F=EDsica. Centro de Ingenier=EDa Gen=E9tica y Biotecnolog=EDa, apartado postal 6162, La Habana, Cuba.Telf: (53-7) 21 8164, 21 8466; Fax: (53-7) 33 6008, 21 8070; E-mail: miriela.gil@cigb.edu.cu TI Estudio comparativo mediante diferentes soluciones para eluir el interfer=F3n alfa recombinante de una columna de inmunoafinidad DE recombinant ? IFN DE elution DE IFN DE immunoaffinity chromatography SO Biotecnologia Aplicada 1998;15:30-33 AB Three solutions were tested to elute the recombinant human alpha 2b interferon (IFN), from an affinity column containing monoclonal antibodies CB IFN 2.4: 1) glycine - NaCl - HCl, 2) acetic acid - NaCl and 3) urea - NaCl - acetic acid. The best performance was obtained with the solution containing urea. The protein was eluted at a higher pH with a higher resolution process and a recovery of the product 18 % greater than the HCl - glycine solution. The presence of urea at low concentrations in the elution modified the antigen-antibody interaction and avoided the aggregation between the IFN molecules. This allowed to concentrate the resulting IFN solution to values greater than 1.5 mg/mL without precipitation of the protein. CC Article on Techniques CC Language:Spanish AU Alberto Mantovani IN Instituto di Ricerche Farmacologiche "Mario Negri", Via Eritrea 62-20157 Milan, Italy and Dept. of Biotechnology, Section of General Pathology, University of Brescia, Italy. TI Novel Pathways for Negative Regulation of Inflammatory Cytokines SO Biotecnologia Aplicada 1998;15:35 CC Report CC Language:English AU Kendall A Smith IN The New York Hospital-Cornell Medical Center, 525 East 68th Street, New York, New York, 10021. USA TI Therapeutic Applicatlons of the Interleukins SO Biotecnologia Aplicada 1998;15:36 CC Report CC Language:English AU James W Larrick, Susan C Wright IN Palo Alto Institute of Molecular Medicine; 2462 Wyandotte Street; Mountain View, CA 94043 USA email; jwlarrick@aol.com TI Molecular Mechanisms of Apoptosis SO Biotecnologia Aplicada 1998;15:37 CC Report CC Language:English AU Robert Whalen IN Director of Research, French National Center of Scientific Research, Paris, France, E-mail: whalen@pasteur.fr The ONA Vaccine Web: http://www.genweb.com/Dnavax/ TI DNA Vaccines: New Applications of Biotechnology to Infectious Diseases SO Biotecnologia Aplicada 1998;15:38 CC Report CC Language:English AU Jos=E9 V Torres, Maria P Carlos IN Medical Microbiology and Immunology, School of Medicine, University of California, Davis, CA 95B16, USA. TI New Approaches for the Design of AIDS Vaccines SO Biotecnologia Aplicada 1998;15:39-40 CC Report CC Language:English AU Quentin-Millet MJ, Rokbi B, Mignon M, Maitre-Wilmotte G, Danve B, Renauld G, Lissolo L IN Pasteur M=E9rieux Connaught, 1541, avenue Marcel M=E9rieux, 69280 Marcy l'Etoile C=E9dex - France TI Transferrin-Binding Proteins as Candidates for a Broadly Cross-Reactive Vaccine Against Serogroup B Meningococcal Disease SO Biotecnologia Aplicada 1998;15:41 CC Report CC Language:English AU Einar Rosenqvist IN Dept. of Vaccinology, National Inst. of Public Health, PO Box 4404 Torshov, N-0403 Oslo, Norway TI Vaccines Against Group B Meningococcal Disease - Problems and Perspeetives SO Biotecnologia Aplicada 1998;15:42 CC Report CC Language:English AU Comit=E9 Internacional de Editores de Revistas M=E9dicas TI Requisitos uniformes para los manuscritos enviados a revistas biom=E9dicas SO Biotecnologia Aplicada 1998;15:43-55 CC Focus CC Language:Spanish From owner-contents@net.bio.net Tue Mar 17 22:00:00 1998 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.journals.contents Subject: Blood Cells, Molecules, & Diseases, vol. 24, no. 05, 15 March 1998 Date: 17 Mar 1998 18:12:36 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 41 Sender: daemon@net.bio.net Approved: biosci-help@net.bio.net Distribution: world Message-ID: <6enaik$i1r@net.bio.net> NNTP-Posting-Host: net.bio.net AU Ida-H. Rennert-OM. Ito-T. Maekawa-K. Eto-Y. TI Type 1 Gaucher Disease: Phenotypic Expression and Natural History in Japanese Patients SO Blood-Cells-Mol-Dis. 1998 Mar 15. 24(5). P. 73-81. DE Gaucher disease DE phenotype DE natural history AB Gaucher disease is caused by a deficiency of glucocerebrosidase, resulting in hepatosplenomegaly, pancytopenia, growth retardation and skeletal involvement. We analyzed data on genotype and key clinical parameters in 35 Japanese patients with Gaucher disease type 1. Our data demonstrated that over 60% of patients had onset of Gaucher disease signs/symptoms at less than 5 years. Sixty percent and 46% of evaluable patients were splenectomized and developed severe bone involvement, respectively. Within mean follow-up periods of 8 years and 4 months, mean relative height and weight, severity score index and platelet count all worsened to a highly significant degree. These data suggest that type 1 Gaucher disease tends to be severe and progressive in Japanese patients, most of whom would be suitable for treatment and might indeed require earlier and more aggressive therapy. CC Reprint request to: Hiroyuki Ida, M.D., Department of CC Pediatrics, Jikei University School of Medicine, 3-25-8 CC Nishi-shinbashi, Minato-ku, Tokyo 105 Japan, CC phone 81-3-3433-1111 ext.3327, fax 81-3-3435-8665, CC email: passion@blue.ocn.ne.jp AU Beutler-E. TI Commentary: The Natural History of Gaucher Disease SO Blood-Cells-Mol-Dis. 1998 Mar 15. 24(5). P. 82-82. DE No keywords AB No abstract CC Reprint request to: Ernest Beutler, M.D., CC The Scripps Research Institute, Department of Molecular and CC Experimental Medicine, 10550 North Torrey Pines Road, CC La Jolla, CA 92037, phone(619)784-8040, fax (619)784-2083, CC email: beutler@scripps.edu