From owner-contents@net.bio.net Wed Oct 27 19:03:00 1999 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.journals.contents Subject: Diseases of Aquatic Organisms, vol. 38, no. 01, 11 October 1999 Date: 27 Oct 1999 13:03:49 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 79 Sender: daemon@net.bio.net Approved: biosci-help@net.bio.net Distribution: world Message-ID: <7v7lr5$2dc@net.bio.net> NNTP-Posting-Host: net.bio.net CC Inter-Research Science Publisher CC Internet Service CC Diseases of Aquatic Organisms Volume 38, Number 1, table of contents CC ------------------------------------------------------------------- CC Abstracts from Inter-Research journals, along with a searchable CC index, are available on the Internet (http://www.int-res.com). CC Contents are also sent to a mailing list (contents@int-res.com). CC Inter-Research, Nordbuente 23, D-21385 Oldendorf/Luhe, Germany CC Tel: (+49)(0) 4132 7127 CC Fax: (+49)(0) 4132 8883 CC E-mail: ir@int-res.com CC Copyright Inter-Research, Oldendorf/Luhe, 1999 CC ------------------------------------------------------------------- CC ------------------------------------------------------------------- CC Diseases of Aquatic Organisms (ISSN 0177-5103) CC Volume 38, Number 1 (1999) CC October 11 CC ------------------------------------------------------------------- CC RESEARCH ARTICLES AU Sukhumsirichart-W. Wongteerasupaya-C. Boonsaeng-V. Panyim-S. Sriurairatana-S. Withyachumnarnkul-B. Flegel-T-W. TI Characterization and PCR detection of hepatopancreatic parvovirus (HPV) from Penaeus monodon in Thailand. SO Dis-Aquat-Org. 1999 Oct 11. 38(1). P DAO 38:1-10. AU Harris-L-J. Owens-L. TI Production of exotoxins by two luminous Vibrio harveyi strains known to be primary pathogens of Penaeus monodon larvae. SO Dis-Aquat-Org. 1999 Oct 11. 38(1). P DAO 38:11-22. AU Senson-P-R. Stevenson-R-M-W. TI Production of the 57 kDa major surface antigen by a non-agglutinating strain of the fish pathogen Renibacterium salmoninarum. SO Dis-Aquat-Org. 1999 Oct 11. 38(1). P DAO 38:23-31. AU Dykova-I. Figueras-A. Novoa-B. TI Epizoic amoebae from the gills of turbot Scophthalmus maximus. SO Dis-Aquat-Org. 1999 Oct 11. 38(1). P DAO 38:33-38. AU Lom-J. Dykova-I. Tonguthai-K. TI Kabataia gen. n., a new genus proposed for Microsporidium spp. infecting trunk muscles of fishes. SO Dis-Aquat-Org. 1999 Oct 11. 38(1). P DAO 38:39-46. AU Sangmaneedet-S. Smith-S-A. TI Efficacy of various chemotherapeutic agents on the growth of Spironucleus vortens, an intestinal parasite of the freshwater angelfish. SO Dis-Aquat-Org. 1999 Oct 11. 38(1). P DAO 38:47-52. CC REVIEW AU Van-Bressem-M-F. Van-Waerebeek-K. Raga-J-A. TI A review of virus infections of cetaceans and the potential impact of morbilliviruses, poxviruses and papillomaviruses on host population dynamics. SO Dis-Aquat-Org. 1999 Oct 11. 38(1). P DAO 38:53-65. CC NOTES AU Otta-S-K. Shubha-G. Joseph-B,Chakraborty-A. Karunasagar-I. Karunasagar-I. TI Polymerase chain reaction (PCR) detection of white spot syndrome virus (WSSV) in cultured and wild crustaceans in India. SO Dis-Aquat-Org. 1999 Oct 11. 38(1). P DAO 38:67-70. AU Nagelkerken-I. Smith-G-W. Snelders-E. Karel-M. James-S. TI Sea urchin Meoma ventricosa die-off in Curacao (Netherlands Antilles) associated with a pathogenic bacterium. SO Dis-Aquat-Org. 1999 Oct 11. 38(1). P DAO 38:71-74. AU McKibben-C-L. Pascho-R-J. TI Shedding of Renibacterium salmoninarum by infected chinook salmon Oncorhynchus tschawytscha. SO Dis-Aquat-Org. 1999 Oct 11. 38(1). P DAO 38:75-79. From owner-contents@net.bio.net Wed Oct 27 19:06:00 1999 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.journals.contents Subject: Aquatic Microbial Ecology, vol. 19, no. 03, 27 October 1999 Date: 27 Oct 1999 13:06:54 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 75 Sender: daemon@net.bio.net Approved: biosci-help@net.bio.net Distribution: world Message-ID: <7v7m0u$2oh@net.bio.net> NNTP-Posting-Host: net.bio.net CC Inter-Research Science Publisher CC Internet Service CC Aquatic Microbial Ecology Volume 19, Number 3, table of contents CC ------------------------------------------------------------------- CC Abstracts from Inter-Research journals, along with a searchable CC index, are available on the Internet (http://www.int-res.com). CC Contents are also sent to a mailing list (contents@int-res.com). CC Inter-Research, Nordbuente 23, D-21385 Oldendorf/Luhe, Germany CC Tel: (+49)(0) 4132 7127 CC Fax: (+49)(0) 4132 8883 CC E-mail: ir@int-res.com CC Copyright Inter-Research, Oldendorf/Luhe, 1999 CC ------------------------------------------------------------------- CC ------------------------------------------------------------------- CC Aquatic Microbial Ecology (ISSN 0948-3055) CC Volume 19, Number 3 (1999) CC October 27 CC ------------------------------------------------------------------- CC RESEARCH ARTICLES AU Guixa-Boixereu-N. Vaque-D. Gasol-J-M. Pedros-Alio-C-P. TI Distribution of viruses and their potential effect on bacterioplankton in an oligotrophic marine system. SO Aquat-Microb-Ecol. 1999 Oct 27. 19(3). P 205-213. AU Ducklow-H. Carlson-C. Smith-W. TI Bacterial growth in experimental plankton assemblages and seawater cultures from the Phaeocystis antarctica bloom in the Ross Sea, Antarctica. SO Aquat-Microb-Ecol. 1999 Oct 27. 19(3). P 215-227. AU Carlson-C-A. Bates-N-R. Ducklow-H-W. Hansell-D-A. TI Estimation of bacterial respiration and growth efficiency in the Ross Sea, Antarctica. SO Aquat-Microb-Ecol. 1999 Oct 27. 19(3). P 229-244. AU Reitner-B. Herzig-A. Herndl-G-J. TI Dynamics in bacterioplankton production in a shallow, temperate lake (Lake Neusiedl, Austria): evidence for dependence on macrophyte production rather than on phytoplankton. SO Aquat-Microb-Ecol. 1999 Oct 27. 19(3). P 245-254. AU Lebaron-P. Servais-P. Troussellier-M. Courties-C. Vives-Rego-J. Muyzer-G. Bernard-L. Guindulain-T. Schaefer-H. Stackebrandt-E. TI Changes in bacterial community structure in seawater mesocosms differing in their nutrient status. SO Aquat-Microb-Ecol. 1999 Oct 27. 19(3). P 255-267. AU Wulff-A. Nilsson-C. Sundbaeck-K. Waengberg-S-A. Odmark-S. TI UV radiation effects on microbenthos-a four month field experiment. SO Aquat-Microb-Ecol. 1999 Oct 27. 19(3). P 269-278. AU Newbold-R-W. Jensen-P-R. Fenical-W. Pawlik-J-R. TI Antimicrobial activity of Caribbean sponge extracts. SO Aquat-Microb-Ecol. 1999 Oct 27. 19(3). P 279-284. AU Chung-W-K. King-G-M. TI Biogeochemical transformations and potential polyaromatic hydrocarbon degradation in macrofaunal burrow sediments. SO Aquat-Microb-Ecol. 1999 Oct 27. 19(3). P 285-295. AU Battin-T-J. Butturini-A. Sabater-F. TI Immobilization and metabolism of dissolved organic carbon by natural sediment biofilms in a Mediterranean and temperate stream. SO Aquat-Microb-Ecol. 1999 Oct 27. 19(3). P 297-305. CC NOTE AU Cantin-G. Levasseur-M. Schultes-S. Michaud-S. TI Dimethylsulfide (DMS) production by size-fractionated particles in the Labrador Sea. SO Aquat-Microb-Ecol. 1999 Oct 27. 19(3). P 307-312. From owner-contents@net.bio.net Wed Oct 27 19:11:00 1999 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.journals.contents Subject: Biotecnologia Aplicada, vol. 16, no. 04, 1999 Date: 27 Oct 1999 13:11:10 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 427 Sender: daemon@net.bio.net Approved: biosci-help@net.bio.net Distribution: world Message-ID: <7v7m8u$39d@net.bio.net> NNTP-Posting-Host: net.bio.net CC BIOTECNOLOGIA APLICADA CC Vol. 16 No. 4 (October-December, 1999) CC ISSN 0864-4551 (printed) CC ISSN 1027-2852 (electronic) CC Copyright 1999, Elfos Scientiae. CC Founded in 1983 and published as Interferon y CC Biotecnologia until 1990, Biotecnologia Aplicada is a CC peer-reviewed scientific journal. It appears four times CC per year, sponsored by the Ibero-Latin-American Society CC of Biotechnology Applied to Health. CC BIOTECNOLOGIA APLICADA is a vehicle of Iberian-Latin- CC American scientists working in different fields of modern CC biotechnology for the diffusion of their experiences and CC results. Some of the main topics that are considered for CC publication are the following: CC a) Molecular genetics and recombinant DNA technology; CC b) Therapeutic and prophylactic uses of molecules CC obtained by biotechnological methods; CC c) Immunotechnology and development of diagnostic CC methods; CC d) Chemistry, biochemistry and design of molecules with CC biological activity; CC e) Application of recombinant DNA techniques and its CC products in agriculture and animal science; CC f) Transgenesis; CC g) Fermentation, recovery and purification of biotech- CC nological products; CC h) Scaling-up, plant design and economic evaluation; CC i) Control and automation of processes. CC All rights reserved. No part of this publication may be CC reproduced, stored or transmitted in any form or by any CC means without the prior permission of the Publisher. By CC submitting a manuscript the authors agree that the CC copyright for their article is transferred to the CC Publisher if and when the article is accepted for CC publication. The points of view expressed in the Journal CC are exclusive responsibility of the authors. CC This journal is indexed by the following publications: CC Biosis, Current Biotechnology Abstracts, Derwent Biotech- CC nology Abstracts, Cambridge Scientific Abstracts, CC Chemical Abstracts, Biological Abstracts, EMBASE/Excerpta CC Medica, Elsevier BIOBASE/Current Awareness in Biological CC Sciences, LilaCS, Periódica, Bio-Journals (Internet). CC Correspondence and subscriptions CC Ave. 31 entre 158 y 190, CC Cubanacan, Playa CC Ciudad de La Habana, CC Apdo. 6072, Habana 6, Cuba. CC Telephones: (53-7) 33 1917 / 21 8466 / 21 8164 CC Fax: (53-7) 33 1917 / 21 8070 / 33 6008 CC E-mail: elfos.scientiae@cigb.edu.cu CC Full papers are available online through BIOLINE System CC (Internet) through the WWW or by e-mail. Annual subscrip- CC tion or single document purchase are possible. Contact CC the following for further information: CC URL:http://www.bd.org.br/bioline/ CC E-mail address: bio@biostrat.demon.co.uk AU Rosa A Serra, Maureen McDonnell, April Bragg IN Department of Molecular and Cellular Physiology. University of Cincinnati School of Medicine. Cincinnati, OH 45267, USA. E-mail: serrar@email.uc.edu Department of Cell Biology, Vanderbilt University Medical Center. Nashville, TN, USA 37232. TI Transforming Growth Factor-beta in Development and Disease DE BMP DE differentiation DE mechanism of action DE signaling DE Smad DE TGF SO Biotecnologia Aplicada 1999;16:205-218 AB The transforming growth factor-beta (TGF-beta) superfamily consists of multifunctional peptides that control many aspects of cell growth and differentiation. Because of the multifunctional nature of the TGF-betas, an understanding of the mechanism of TGF-beta action should provide significant information regarding the pathogenesis of many diseases. This review will focus on the role and mechanisms of TGF-beta signaling in the mammary gland, skin, lung, and skeleton and will discuss how alterations in TGF-beta signaling may result in disease. CC Review article CC Language: English AU Nieves Santos, Jorge Aguiar, Julio Fernandez, Maria Vazquez, George Auburger, Suzana Gispert, Yssel Mendoza, Julia Garcia, Luis Velazquez IN Department of Neurology. Lenin Hospital. Holguin, Cuba. Department of Pharmaceutics. Center for Genetic Engineering and Biotechnology. PO Box 6162, Havana 10600, Cuba. Phone (53-7) 21 8008; 336008; E-mail: jaguiar@cigb.edu.cu Department of Neurology. University Hospital. Düsseldorf, Germany. TI Molecular diagnosis of a sample of the Cuban population with spinocerebellar ataxia type 2 DE CAG repeat expansions DE disease DE spinocerebellar ataxia 2 SO Biotecnologia Aplicada 1999;16:219-221 AB CAG repeat unstable expansions causing spinocerebellar ataxia 2 (SCA2) disease were localized into a CpG island on the exon 1 of the SCA2 gene, previously linked to the chromosomic region 12q23-24.1 of human chromosome 12. At molecular level, 392 patients with dominant ataxia from Holguin, Cuba, were analyzed, showing an inverse correlation between CAG repeat length and the age of disease onset. The smallest CAG repeat expansion causing neurodegeneration among the SCA2 patients was also found. For SCA2 patients with (CAG)n stretches of 32 to 40 repeats, there is a high variability with respect to the age of disease manifestation. These findings suggest that for each patient other specific genetic and/or environmental factors are very significant for the begining and evolution of the disease for small sizes of CAG repeat expansions. CC Research Article CC Language: Spanish AU Angel Valdivia, Danay Chacon, Clara Savon, Odalys Valdes, Grehete Gonzalez, Reynel Cancio, Julio C Sanchez, German Roges, Blanca Garcia-Barreno, Jose A Melero, Angel Goyenechea IN Deparment of Virology. Institute of Tropical Medicine "Pedro Kouri". PO Box 601, Marianao 13, Havana, Cuba. Fax: (53-7) 24 6051, 22 0633; E-mail: a.valdivia@ipk.sld.cu Center for Genetic Engineering and Biotechnology. PO Box 6162. Havana 6, Cuba. National Center of Fundamental Biology, Majadahonda 28220 Madrid, Spain. TI Confirmation of Unusual Epidemiological Behavior of the Human Respiratory Syncytial Virus During Two Consecutive Epidemics (Seasons 94/95 and 95/96) in Havana City, Cuba DE HRSV DE molecular epidemiology DE respiratory syncytial virus SO Biotecnologia Aplicada 1999;16:222-225 AB Twenty-seven strains of the human respiratory syncytial virus, isolated from three consecutive outbreaks, were sequenced at the National Center of Fundamental Biology (Madrid, Spain). The nucleotide sequence showed two unique characteristics related to what had been previously reported in the literature: (i) all of them were identical, and (ii) all showed a great homology with the Spanish Long reference strain (with only five changes). Upon this unusual finding and with the main objective of excluding a probable contamination with another Long strain from Sweden, selected regions of both reference strains and other laboratory strains were sequenced. The Spanish Long strain was only used as a confirmatory control of the results obtained in laboratories from Spain. The results obtained by the authors discarded any possible contamination when the percentages of homology between the sequenced regions of the two foreign strains and the Cuban isolates were analyzed and compared. The results also corroborated that the Swedish Long strain was even more separated from the Cuban isolates than the Spanish strain. This report presents a theory to explain the unusual epidemiological behavior of this virus in Cuba. CC Research article CC Language: English AU Santiago Dueñas-Carrera, Juan Morales, Nelson Acosta-Rivero, Lazaro J Lorenzo, Ciro Garcia, Thelvia Ramos, Ivis Guerra, Maxlenin Peña IN Centro de Ingenieria Genetica y Biotecnologia. AP 6162, CP 10600, Habana, Cuba. Fax: (53-7) 214764; E-mail: juan.morales@cigb.edu.cu TI Variable Level Expression of Hepatitis C Virus Core Protein in a Prokaryotic System. Analysis of the Humoral Response against It in Rabbits DE E. coli DE expression DE HCV DE hepatitis C virus DE immunogenicity DE nucleocapsid core protein DE purification SO Biotecnologia Aplicada 1999;16:226-231 AB Experimental evidence suggests that the hepatitis C virus (HCV) core protein has several biological properties and is implicated as a viral factor in HCV-mediated pathogenesis. In this study, CoE1.339, Co.176 and Co.120 variants of the HCV capsid protein were produced at variable levels in Escherichia coli from pNCoE1, pN12 and pSLCo120 plasmids, respectively. The chimera CoE1.339 spans the first 339 amino acids of the viral polyprotein, fused to the 45 amino acids stabilizer peptide from P64K protein of Neisseria meningitidis. Co.176 is a non-fused variant encompassing amino acids 1-176 of the capsid protein sequence. Both CoE1.339 and Co.176 were expressed under the transcriptional control of the tryptophan promoter and the protein was only detected by Western blot. However, Co.120, a variant truncated at the C terminus which contains amino acids 1-120 of the HCV core protein fused to a leader tag of six histidines, was successfully produced under the control of the T7 promoter in BL21 (DE3) cells. Like Co.176 and CoE1.339, Co.120 was efficiently recognized by human anti-HCV positive serum. Purified Co.120 antigen showed to be immunogenic in rabbits. Synthetic peptides covering amino acids 1-40 of the core protein showed the greatest reactivity to the rabbit anti-Co.120 serum. CC Research Article CC Language: English AU Diana Garcia del Barco, Alina Rodriguez, Elsa Rodriguez, Caridad Tamayo, Julio R Fernandez, Maria M Vasquez, Maria P Rodriguez, Ricardo Lleonart IN Division de Genetica de Celulas de Mamiferos. Centro de Ingenieria Genetica y Biotecnologia. AP 6162, CP 10600, La Habana, Cuba. Telf: (53-7) 21 8164; Fax: (53-7) 33 6008; E-mail: diana.garcia@cigb.edu.cu TI Erythropoietin gene co-amplification in methotrexate-resistant Chinese hamster ovary dhfr- cells. DE CHO DE EPO DE erythropoietin DE gene amplification SO Biotecnologia Aplicada 1999;16:232-235 AB Gene amplification plays an important role in cellular regulatory mechanisms. To study the amplification and expression of the human erythropoietin (EPO) gene, Chinese hamster ovary (CHO) dhfr- cells co-transfected with chimeric genes for the expression of EPO and dihydrofolate reductase (DHFR) and selected in the presence of increasing concentrations of methotrexate, were used. The results evidenced a three-fold gene amplification, accompanied by an increase in EPO expression of up to 33 pg/cell/day. The amplicon, which contains host genomic sequences, shows rearrangements originated during the integration-amplification process CC Research Article CC Language: Spanish AU Lisset Herrera, Orlene Guerra, Pedro L Ramos, Rudy Peral, Ana L Echemendia, Nadia Ramirez, Vivian Doreste, Pedro Oramas IN Laboratory of Biochemistry. Bioplant Center. University of Ciego de Avila. Carretera a Moron Km 9,5. CP 69450. Ciego de Avila, Cuba. Plant Biotechnology Division. Center for Genetic Engineering and Biotechnology. PO Box 6162, Havana, Cuba. Fax: (53-7) 21 8070; E-mail: Pedro.Oramas@cigb.edu.cu Instituto de Sanidad Vegetal. Playa, Ciudad de La Habana, Cuba. TI Molecular Techniques for the Detection of Tomato Yellow Leaf Curl Geminivirus in Infected Plants and Viruliferous Whiteflies DE Dot blot DE geminiviruses DE PCR DE TYLCV DE virus diagnosis SO Biotecnologia Aplicada 1999;16:237-241 AB The tomato yellow leaf curl virus (TYLCV) causes major yield losses in tomato production in many tropical and subtropical regions. Therefore, there is an increased need to apply molecular methods for the detection and characterization of the main TYLCV isolates that affect Cuban tomato plantations. A DNA fragment containing the gene encoding the coat protein of a Cuban TYLCV isolate was amplified by polymerase chain reaction (PCR). DNA hybridization with specific radiolabeled probes corroborated the identity of the amplified product, and allowed to analyze the integration into a full-length TYLCV genome. The threshold of TYLCV detection by PCR in plants and viruliferous whiteflies was within the reported range. To detect TYLCV infections, samples collected from tomato plantations of several regions in Cuba were analyzed by PCR. The amplified coat protein gene was also effectively used as a DNA probe in Dot blot assays to detect geminivirus in plants CC Paper on Techniques CC Language: English AU Guillermo Pratta, Roxana Zorzoli, Liliana A Picardi IN Catedra de Genetica. Facultad de Ciencias Agrarias. Universidad Nacional de Rosario. CC 14, 2123 Zavalla, Argentina. Telf: (54-0341) 497 0080; Fax: (54-0341) 497 0085; E-mail: gpratta@fcagr.unr.edu.ar TI Obtainment and micropropagation of intra- and interspecific hybrids of tomato (genus lycopersicon) DE embryo rescue DE in vitro plant tissue culture DE plant breeding DE plant genetic resources SO Biotecnologia Aplicada 1999;16:242-245 AB In vitro plant tissue culture is a useful tool for overcoming the problems in obtaining hybrids (i.e., embryo abortion, production of seeds) when wild species are incorporated to breeding programs. The ability for producing intra- and interspecific hybrids among different crosses in the genus Lycopersicon, the adequacy of in vitro culture of immature embryos to break the unilateral incompatibility, and the micropropagation response of the hybrids obtained, were evaluated. Different genotypes of the species and varieties of L. esculentum, L. esculentum var. cerasiforme, L. pimpinellifolium, L. peruvianum, and L. hirsutum, were used. Differences in the ability to produce intra- and interspecific hybrids were found among the crosses. In vitro culture of immature embryos was successful in obtainintg interspecific hybrids when the crosses presented unilateral incompatibility. In this case, there also were differences in the ability to cross among the genotypes. The capacity for in vitro regeneration of the intra- and interspecific hybrids originated from compatible crosses was in general superior to that of the parental lines, but a decrease in the regeneration capacity was observed in the hybrids originated from incompatible crosses, with respect to the parental genotypes. CC Paper on Techniques CC Language: Spanish AU Mauro Alfonso, Peter Ifversen, Jesper Zeuthen IN Center of Molecular Immunology. PO Box 16040, Havana 11600, Cuba. E-mail: mauro@ict.cim.sld.cu Department of Tumor Cell Biology. Danish Cancer Society, DK-2100 Copenhagen, Denmark. TI Human Anti-ganglioside IgG Antibody Response Induced by in vitro Immunization with Liposomes Containing GM3(NeuGc) Ganglioside DE ganglioside DE human antibody DE in vitro immunization SO Biotecnologia Aplicada 1999;16:246-248 AB A new in vitro immunization system of human peripheral blood lymphocytes using liposomes containing gangliosides, is described. Purified human B lymphocytes were cultured in the CD40 system in the presence of liposomes containing different concentrations of GM3(NeuGc) ganglioside, ranging from 1 to 1000 ng/mL. After an initial primary in vitro immunization period (3 4 days), antigen-stimulated B cells and autologous activated CD4+ T lymphocytes previously activated with an anti-CD3 monoclonal antibody were cultured in the presence of staphylococcal enterotoxin A. Human antibody production was measured by an anti-ganglioside ELISA after 4 and 8 days of culture. Specific antibody responses against GM3(NeuGc) of IgM and IgG isotypes were induced after 4 days in culture, and a predominant antigen-specific IgG antibody production was observed 8 days after antigenic stimulation. CC Article on Techniques CC Language: Spanish AU Gerardo Guillen IN Centro de Ingenieria Genetica y Biotecnologia. Ave. 31 entre 158 y 190, Cubanacan, Playa. AP 6162, CP 10600, Cuba. Telf: (53-7) 21 6221; Fax: (53-7) 21 4764; E-mail: gerardo.guillen@cigb.edu.cu TI XI Congreso Internacional de Virologia SO Biotecnologia Aplicada 1999;16:249-252 CC Report CC Language: Spanish AU Gerardo Guillen IN Centro de Ingenieria Genetica y Biotecnologia. Ave. 31 entre 158 y 190, Cubanacan, Playa. AP 6162, CP 10600, Cuba. Telf: (53-7) 21 6221; Fax: (53-7) 21 4764; E-mail: gerardo.guillen@cigb.edu.cu TI IX Congreso Internacional de Bacteriologia y Micologia SO Biotecnologia Aplicada 1999;16:253-255 CC Report CC Language: Spanish AU Maria L Genta, Hugo D Genta IN Laboratorio de Tecnologia de Alimentos, Facultad de Bioquimica, Quimica y Farmacia. Instituto de Ingenieria Quimica. Facultad de Ciencias Exactas y Tecnologia. Universidad Nacional de Tucuman. Ave. Independencia 1800, 4000 San Miguel de Tucuman, Argentina. Telf: (54-381) 436 4093 (Interno 222); Fax: (54-381) 436 3004; E-mail: nalvarez@herrera.unt.edu.ar TI La bioindustria en Japon SO Biotecnologia Aplicada 1999;16:257-260 CC Focus CC Language: Spanish AU Jorge Bacallao, Josefina Lugo IN Dpto. Computacion y Biometria. Instituto Superior de Ciencias Medicas. Calle 146 No. 3102, La Habana 11600, Cuba. E-mail: bacallao@comput.giron.sld.cu Dpto. Ensayos Clinicos. Instituto Nacional de Oncologia y Radiobiologia. Ciudad de La Habana, Cuba. TI Use of prior Knowledge in Randomized Clinical Trials with a Binary Response Variable DE beta-binomial distribution DE binary response DE clinical trials DE non-Bayesian methods SO Biotecnologia Aplicada 1999;16:261-263 AB When testing a new drug, the researcher faces the ethical and technical problem of incorporating the existing knowledge derived from previous trials, from a meta-analysis or simply from his subjective judgement. This paper proposes a non-Bayesian method based on the beta-binomial distribution, which can be used in the clinical trial of a drug or treatment when the response variable is dichotomous. The method consists in the modeling of the previous knowledge by means of the beta distribution in the assessment of both toxicity and efficacy, and a statistical test of efficacy, which depends on a parameter of the beta-binomial distribution. CC Focus CC Language: Spanish