From mduhaime from mpi-bremen.de Tue Feb 3 10:30:09 2009 From: mduhaime from mpi-bremen.de (Melissa Beth Duhaime) Date: Tue Feb 3 16:39:06 2009 Subject: [Bio-www] BPROM question Message-ID: <49886301.3000001@mpi-bremen.de> Dear Victor (or whomever may be at the other end of this email...), Thanks for the great software at softberry :) I have been exploring BPROM to predict promoters 150 bp upstream of various predicted ORFs, but I have a question I still cannot resolve. Can you give me any advice, or where to look, to determine an acceptable linear discriminant function threshold by which I should consider the predicted promoters true positives? Is there an accepted norm for BPROM or the application of linear discrimination here? Thanks very much for your help! Cheers, Melissa Duhaime