What is a good DAPI concentration for F.I.S.H. ?
mike.stacey at pharmacology.oxford.ac.uk
Thu May 2 05:53:35 EST 1996
we use a stock concentration of DAPI at 10mg/ml. from this stock I take
1.5ul into 1ml of antifade solution. The antifade i use is called
Vectashield, I can give you details if you want. I spot 10ul of DAPI in
antifade onto a slide and drop on a coverslip and seal with nailvarnish.
Store your slides in the dark. Hope it goes ok, and good luck.
ps I'm trying to do FISH on histological sections, and am having great
difficulty. Have you any experience in this area??
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