From davhak2000 at hotmail.com  Wed Jun  9 03:13:20 2004
From: davhak2000 at hotmail.com (Davit Hakobyan)
Date: Mon Mar  7 05:07:56 2005
Subject: Question about Phosphoglycerate mutase (PGM) mechanism
Message-ID: <538f3dee.0406080209.26d745b4@posting.google.com>


Hi All,

In glycolysis the phosphoglycerate mutase (PGM) converts 3PG into 2PG.
As it appears the phosphorylated HIS residue (8) "gives" its PO3 to
3PG and some B element (probably Boron) takes the Hydrogen atom, which
have been previously bound to the oxygen of the second carbon (C2). My
question is what is this B element, which takes the hydrogen ?
Is it really a Boron ? Because I have couple yeast PGMs (downloaded
from Protein Data Bank site) and they do not have any Boron atom.
The PGM i'm interested the most has its ID 1QHF at PDB (and no Boron
(B) atom).

Thanks for any help.


From sphinx05 at infonie.fr  Wed Jun  9 03:13:52 2004
From: sphinx05 at infonie.fr (Raphael Chaleil)
Date: Mon Mar  7 05:07:56 2005
Subject: Question about Phosphoglycerate mutase (PGM) mechanism
References: <538f3dee.0406080209.26d745b4@posting.google.com>
Message-ID: <ca4k1t$qoc$1@sparta.btinternet.com>


Davit Hakobyan wrote:
> Hi All,
> 
> In glycolysis the phosphoglycerate mutase (PGM) converts 3PG into 2PG.
> As it appears the phosphorylated HIS residue (8) "gives" its PO3 to
> 3PG and some B element (probably Boron) takes the Hydrogen atom, which
> have been previously bound to the oxygen of the second carbon (C2). My
> question is what is this B element, which takes the hydrogen ?
> Is it really a Boron ? Because I have couple yeast PGMs (downloaded
> from Protein Data Bank site) and they do not have any Boron atom.
> The PGM i'm interested the most has its ID 1QHF at PDB (and no Boron
> (B) atom).
> 
> Thanks for any help.
I suppose B stands for a basic group as proton acceptor, nothing to do 
with Boron.

Regards