background problems with Abs

Terese Rakow rakow at SCRIPPS.EDU
Sat Apr 22 11:47:05 EST 1995

>>Has preincubating antibodies with fixed worms removed
>>contaminating Abs?

I have used acetone crystals of bacteria and mutants to clean up antisera
for westerns, this has worked fine.  I basically follow the same protocol
for bacteria in the Harlowe antibody book.  I have not used it for staining
worms though.  

Terese Rakow

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