From luckydenno from gmail.com Tue Jul 3 10:06:50 2007 From: luckydenno from gmail.com (Muhanguzi Dennis) Date: Tue Jul 3 11:05:54 2007 Subject: [Celegans] C.elegans trans-splicing Message-ID: <59b94f170707030806m5e08752boe77efe5f408831a@mail.gmail.com> Hullo, can you please send me some brief information about transsplicing in c-elegans? Truly, Dr. Dennis Muhanguzi (MAKERERE UNIVERSITY) From gilbertd from net.bio.net Thu Jul 12 00:02:38 2007 From: gilbertd from net.bio.net (Don Gilbert) Date: Sun Jul 15 08:06:53 2007 Subject: [Celegans] Open access to waterflea Daphnia genome Message-ID: <200707120502.l6C52c629695@net.bio.net> Dear Biologists, The arthropod crustacean Daphnia pulex (better known as the water flea) is a unique emerging model organism, straddling the genome reach of vertebrate model organisms such as Mouse, insects such as Drosophila, and nematodes such as Caenorhabditis elegans. Daphnia shares with C. elegans an interesting and rich gene structure and wealth of gene duplications. It has closest gene homology to the insects, Apis and Drosophila. However it has nearly as many unique or strong gene homologies to Mouse as to insects. As a model organism for ecology and environmental research for centuries, Daphnia provides a genome-environment experimental model unlike any other, where its natural freshwater environments are readily mimicked in lab, and environmental stresses can be readily tested in large-scale genomic experiments. A great example of this genome-environment connection comes from Daphnia's well known hemoglobin response, called into play as they rapidly turn blood-red when oxygen is depleted in their ponds. Among Daphnia's many gene duplications, we find a tandem cluster of 8 hemoglobin genes (http://wfleabase.org/genome-summaries/gene-duplicates/). Whether you work with human or vertebrate genomes, insects, worms or other eukaryotes, you will find many aspects of interest in comparing your genomes of interest to this first crustacean draft genome sequence. The Daphnia pulex genome will be available on 2007/07/07, in public early access, with a grand opening scheduled between Fall 2007 and Spring 2008, which will include a Genbank genome submission and publications. These sequence data were produced by the US Department of Energy Joint Genome Institute in collaboration with the Daphnia Genomics Consortium. This early access release is made available via Daphnia Genome Consortium, http://daphnia.cgb.indiana.edu/ The DGC website contains Daphnia community documents, analyses, and summaries of this emerging model organism's value to genomics, ecology, environmental toxicology, evolution, and other areas. JGI Genome Portal, http://genome.jgi-psf.org/Dappu1/ The JGI portal provides gene predictions and homology reports, with services for search, BLAST, Genome map Browser genes categorized by GO, KEGG, KOG classifications and bulk data downloads. Daphnia genome database at wFleaBase.org provides Summaries of gene homology, structure, phylogeny http://wfleabase.org/genome-summaries/ Genome maps, BLAST, Bulk search services at http://wfleabase.org/ Daphnia pulex genome data sets in common bulk formats http://wfleabase.org/genome/Daphnia_pulex/current/ Although the time and effort to bring Daphnia's genome to public access has been large, we believe the quality of this genome data is high and will be valuable to many areas of genome research. Please note the public release status for publication of the JGI and Daphnia Genome Consortium's data, and respect their investment of effort on this new model genome. Scientists wanting to contribute to the open community-wide annotation project that is currently ongoing should visit http://dgc.cgb.indiana.edu -- Don Gilbert for Daphnia Genome Consortium From jdpeders from ncsu.edu Wed Jul 11 12:34:12 2007 From: jdpeders from ncsu.edu (Julie Pederson) Date: Sun Jul 15 08:06:58 2007 Subject: [Celegans] Postdoctoral Research Associate Position Message-ID: <003101c7c3e1$b21866b0$16493410$@edu> Please post the following to the C. elegans listserver: Postdoctoral position - Gene regulatory networks controlling organogenesis in C. elegans An NSF-funded postdoctoral position is available in the Mathies lab at North Carolina State University to study development of the reproductive organs in C. elegans. The postdoctoral fellow will join an ongoing effort to define the gene regulatory network controlling somatic gonad development (http://www.cals.ncsu.edu/genetics/mathies/mathies.html). He/she will employ a variety of molecular genetic approaches to elucidate the functions of novel transcriptional regulators and to fit these regulators into a gene regulatory framework. A Ph.D. in genetics, biology, biochemistry or equivalent is required and experience with protein/DNA interactions or developmental biology is desirable. North Carolina State University is located in the Research Triangle Park (within a half hour drive of Duke University and UNC - Chapel Hill). This setting provides excellent opportunities for career development and synergistic interactions with academic and industrial scientists. In addition, the Triangle has an active C. elegans community with participants from all area institutions (http://celegans.bio.unc.edu/). To apply go to jobs.ncsu.edu/applicants/Central?quickFind=76959 and provide a curriculum vitae, names, phone numbers and e-mail addresses of three references. ______________________________________________________ NC State University is an equal opportunity and affirmative action employer. All qualified applicants will receive consideration for employment without regard to race, color, national origin, religion, sex, age, veteran status, or disability. In addition, NC State University welcomes all persons without regard to sexual orientation. ADA Accommodations: Jeffrey Hawley (jeff_hawley@ncsu.edu; ph: 919-515-5727; fax: 919-515-3355). Julie Douglas Pederson Department of Genetics North Carolina State University Raleigh, NC 27695-7614 Tel: 919-515-4248 Fax: 919-515-3355 julie_pederson@ncsu.edu From fares from email.arizona.edu Mon Jul 16 12:35:22 2007 From: fares from email.arizona.edu (Hanna Fares) Date: Wed Jul 18 11:56:50 2007 Subject: [Celegans] split ubiquitin Message-ID: <23792294-95FF-4C27-9ECA-25E4E5E89A68@email.arizona.edu> Greetings all, I was wondering whether anybody is using the yeast two-hybrid split ubiquitin system and whether you have converted the vectors to the Gateway system. Johnny Hanna (Johnny) Fares Associate Professor University of Arizona Department of Molecular and Cellular Biology Life Sciences South Building, Room 531 1007 East Lowell Street Tucson, AZ 85721 United States of America Office Phone (520) 626-3759 Lab Phone (520) 626-5996 Fax (520) 621-3709 From fares from email.arizona.edu Fri Jul 27 15:29:12 2007 From: fares from email.arizona.edu (Hanna Fares) Date: Fri Jul 27 16:08:26 2007 Subject: [Celegans] split-ubiquitin y2h response Message-ID: <6DC98A05-1C0E-4CD6-BA70-D539635F39A7@email.arizona.edu> Greetings All, I emailed a week ago to ask whether anybody had converted the split- ubiquitin y2h vectors for Gateway cloning. Apparently, nobody in the worm community has. I also contacted a company, DualSystems, that sells a split-ubiquitin y2h kit. The whole kit costs $3500. It seems they have converted their vectors to Gateway by adding attR sites. They are in the process of resolving legal issues with Invitrogen, so I am not sure when these will be on the market. Johnny Johnny Fares Associate Professor University of Arizona Department of Molecular and Cellular Biology Life Sciences South Building, Room 531 1007 East Lowell Street Tucson, AZ 85721 United States of America Office Phone (520) 626-3759 Lab Phone (520) 626-5996 Fax (520) 621-3709 From vsmith from ku.edu Fri Jul 27 14:39:33 2007 From: vsmith from ku.edu (Val Smith) Date: Fri Jul 27 16:08:40 2007 Subject: [Celegans] C. elegans diet Message-ID: <200707271939.l6RJdVZc027316@smtpku1.cc.ku.edu> Dear Colleagues: An undergraduate in my laboratory is interested in working with C. elegans grown in semi-continuous liquid culture. Most published studies that I have found use monocultures of eubacteria as the primary food source; one study used yeast cells. I was wondering whether small unicellular algae (such as the green alga Chlorella) can successfully be used as an alternative food source as well? Many thanks for your kind help! Best regards, Val Val H. Smith Professor Department of Ecology and Evolutionary Biology University of Kansas Lawrence, KS 66045 785-864-4565 FAX: 785-864-5321 e-mail: vsmith@ku.edu Val H. Smith Professor Department of Ecology and Evolutionary Biology University of Kansas Lawrence, KS 66045 785-864-4565 FAX: 785-864-5321 e-mail: vsmith@ku.edu