From g.Joshua from lshtm.ac.uk  Thu Jan  8 07:11:07 2009
From: g.Joshua from lshtm.ac.uk (g.Joshua@lshtm.ac.uk)
Date: Thu Jan  8 12:23:51 2009
Subject: [Celegans] Real Time PCR
Message-ID: <4965ED5A.00D0.0083.0@lshtm.ac.uk>

Dear colleagues,
Is there a consensus on best/good housekeeping gene in C. elegans to use 
to normalise against in Real Time PCR?
Have tried act-3 (see Park et al (2007) J Mol Biol 372 (2), 331-340) but 
find large differences in RNAs from two worm populations (in all replicates).
Best regards,
G. Joshua.
 
 
G.W.P.Joshua
London School of Hygiene and Tropical Medicine
Dept. Infectious and Tropical Diseases,
Keppel St.,
London WC1E 7HT.
Tel:     (+44) (0)20 7927 2028
FAX:    (+44) (0)20 7637 4314
E-mail:  g.joshua@lshtm.ac.uk
From SnyderD2 from niehs.nih.gov  Thu Jan 15 10:57:38 2009
From: SnyderD2 from niehs.nih.gov (Snyder, Daniel (NIH/NIEHS) [E])
Date: Thu Jan 15 11:55:11 2009
Subject: [Celegans] pmyo2::mcherry line?
Message-ID: <0C73F0251F73EE478071849626186D6801FD30F6@NIHCESMLBX6.nih.gov>

Does anyone have an integrated strain expressing mCherry behind the
myo-2 promoter? Our group is interested in using this strain to
investigate the role of toxicant exposure in a motion tracking assay.

 

Daniel Snyder                       Phone: (919) 541-2533

Research Assistant                Fax: (919) 541-1460

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