From g.Joshua from lshtm.ac.uk  Fri Sep 11 06:20:12 2009
From: g.Joshua from lshtm.ac.uk (g.Joshua@lshtm.ac.uk)
Date: Fri Sep 11 10:05:53 2009
Subject: [Celegans] Single worm reverse-transcriptase (RT) PCR
Message-ID: <4AAA407B.00D0.0083.1@lshtm.ac.uk>

Dear colleagues,
I'm advised that putative positives from RNA-interference (RNAi) experiments (using feeding strains from Geneservice {Fraser et al [2000]. Nature 408 (6810) 325-30}) can be checked by RT-PCR. This requires reverse transcriptase reaction on single worms. 
Single worm PCR lysis buffer doesn't seem amenable to reverse transcriptase reaction {?} 
Does anyone have a method/protocol for single worm RT-PCR ?
G. Joshua.
 
 
G.W.P.Joshua
London School of Hygiene and Tropical Medicine
Dept. Infectious and Tropical Diseases,
Keppel St.,
London WC1E 7HT.
Tel:     (+44) (0)20 7927 2028
FAX:    (+44) (0)20 7637 4314
E-mail:  g.joshua@lshtm.ac.uk