CD9 for Granulocytes

[Aultk]

Gail Sullivan writes:
>I have a reference (J Immunol. Meth. 145:105-110; 1991) which uses a
>FITC-conjugated anti-CD9 from Sera Lab, Zurzach, Switzerland to identify
>eos mixed with PMN.  

   I have not read the article you refer to, but I would be concerned that CD9 would label
platelets that are bound, (in highly variable numbers, depending on how you prepare your
cells), to granulocytes.  We have not investigated whether platelets bind to eosinophils, but we
know they bind to resting and activated neutrophils and monocytes.

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 Kenneth A. Ault M.D.
 Maine Medical Center Research Institute
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