Hello, Could somebody help me ? I would like to make an discontinuous gradient of Percoll and Ficoll. I have Ficoll from SEROMED 1.077 g/ml, the Percoll 1.077 g/ml and PBS without Ca and Mg. How many of each density should I put ? Could somebody me send by e-mail the protocole ? Thanks. Anne Jungbltuh jungbluth at orto.ucl.ac.be